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"inoculating" Antonyms

217 Sentences With "inoculating"

How to use inoculating in a sentence? Find typical usage patterns (collocations)/phrases/context for "inoculating" and check conjugation/comparative form for "inoculating". Mastering all the usages of "inoculating" from sentence examples published by news publications.

Nonfiction Power has a way of inoculating itself against dissent.
But just inoculating babies with B. infantis won't be enough.
He used to ride around Deh Naw on a motorcycle, inoculating bedridden villagers.
Anti-vaccination advocates claim that inoculating children can cause diseases or developmental disorders, like autism.
To consider development in terms of God's plan can mean inoculating oneself from its consequences.
Instead, they act more like public health workers, inoculating communities from viral misinformation and its consequences.
During that time, honeybees regurgitate the nectar, inoculating it over and over with their gut bacteria.
Instead, they act more like public health workers, inoculating communities from viral misinformation and its consequences.
That would be Trump's way of paying back Netanyahu for inoculating him against charges of anti-Semitism.
Other research has been done on inoculating children born through C-section with their mothers' vaginal bacteria.
To be sure, there are ways that Mueller can go about inoculating himself from challenges to future actions.
By hammering at federal investigators, Southers said, Trump was inoculating himself from any political fallout that may follow.
But in that conflict, the president may have handed Senate Democrats the whip, while inoculating them from blame.
Simply purchase a koji start at an Asian specialty market and follow the instructions on inoculating your mother culture!
The first is the one you immediately think about: understanding the disease, researching a cure and inoculating the population.
His colleagues initially suggest that this could be a good thing, inoculating the party from accusations of race hate.
"Understanding how these genes might be inoculating these animals from cancer could be a huge benefit to humans," says Stanhope.
One Republican lobbyist said that Google's donations have had an "inoculating effect" now that the political tide is turning against tech.
On the ground, the battle is fought with teams of forestry experts going tree-to-tree inoculating root systems against fungus.
The oral vaccine could eventually be injected into tasty baits that would be scattered throughout ape habitats, inoculating the population over time.
And this is the Achilles's heel of vaccination programs—controlling outbreaks relies on "herd immunity," inoculating a large percentage of the population.
Health authorities have also rolled out the vaccine to tackle the outbreak in North Kivu, inoculating more than 18,000 people since August.
Rio de Janeiro, for one, is aggressively vaccinating its citizens in hopes of inoculating 12 million by the end of the year.
In this case, the strategy comes with an additional benefit: inoculating him from televised attacks from opponents and risking a rhetorical misstep.
Or they may see this as an inoculating vote, a way to show non-partisan points of agreement with Trump in swing districts.
Since students led the 1989 protests that occupied Tiananmen Square in Beijing, party leaders have made a priority of inoculating them against liberal values.
There is a network effect to EMV adoption—widespread retail use of EMV is the equivalent of the industry inoculating itself against certain fraud.
Nurses at one medical center are treating and inoculating kids in the parking lot to reduce the risk of contagion in the waiting room.
But the thought disgusted him, and instead he opened a kitchen drawer and grabbed an inoculating loop, an instrument used by microbiologists to sample microorganisms.
He removed his T-shirt and unscrewed a test tube filled with saline solution and the cotton inoculating swab that Zayner rubbed on Michael's arms.
Another way to shield Mueller would be for prominent figures to promote Mueller's stellar reputation, thereby inoculating against any calumny the Trump team might bring.
But there's good news: A switch to USB-C would bring Apple into an established ecosystem, theoretically inoculating us all against such a messy turnover.
A ring vaccination involves inoculating people who have come into direct contact with individuals confirmed to have Ebola, as well as the contacts of those contacts.
There was a time though ... However, scientists weren't always this united on the folly of nuking, coating, coaxing, inoculating or otherwise warding off mammoth weather events.
The earliest vaccines were primitive – think Edward Jenner in the 1790s inoculating against smallpox by rubbing together the open wounds of uninfected patients and those with cowpox.
"To draw them I use an inoculating needle, and a drop of sterile water in a petri dish mixed with a colony of the bacteria," she said.
If you're idealistic enough to think that we're going to deal in a truthful environment, then you might not have to worry about inoculating against their poison.
The "antidote," as practiced by Girard, was the pulling together of cultural roots from other places and societies, essentially inoculating the sterilized Modernist field with new cultures.
More likely, they are inoculating their brews with selected yeast strains, including brettanomyces, anathema to winemakers as it can be the source of funky flavors great and small.
Pakistan has made huge strides in tackling polio, but officials say that while the latest immunization drive succeeded in inoculating 37.6 million children, 1.4 million were left unprotected.
Thousands vaccinated Since August 8, the Ministry has been inoculating people at high risk of an Ebola infection: primary and secondary contacts of those with confirmed Ebola infections.
In theory, health officials could even act pre-emptively — inoculating a population against a dangerous new flu or coronavirus circulating in animals before it infects many people, for example.
Those recollections, and images of Eichmann supervising the mass slaughter of innocents, serve as cautionary ballast, inoculating the audience against being too caught up in a suspenseful, entertaining wartime thriller.
Used for missions around the world, Green Berets span a continuum of missions from "soft power" — inoculating local children from disease and building rapport with local leaders — to intense combat operations.
Trump is swinging his counterpunches off CNN, NBC, and ABC, preemptively inoculating his audience from potential attacks against his administration — something that worked for him during the primaries and general election.
More than 730,000 people in the Philippines have been vaccinated with Dengvaxia since April 2016, when the World Health Organization first recommended inoculating anyone 9 and older living in high-risk areas.
A 1972 grant from the National Endowment of the Arts enabled Stan to purchase the former bakery that houses it, inoculating it against rising rents that have imperiled other local arts outposts.
The country has engaged in a concerted effort to vaccinate its population ahead of the closure, inoculating more than 85033,000 people with a focus on young children and women of childbearing age.
Firstly how powerful misinformation can be, says van der Linden, but also — on the positive side — that 'inoculating' people against misinformation can be effective even if a person has an entrenched prior viewpoint.
While the Interior secretary asserts increased functionality and efficiency, the truth is that abolishing the BLM's central inoculating functions will render its entire system of public lands more susceptible to isolation and exploitation.
They called it the Resilience Project, and researchers set out to find individuals in the population who carried the DNA variants for those diseases but somehow, through some inoculating buffer, didn't have the disease.
Dawson and his researchers set out to test the rate of bacterial transfer to both lemons and ice by inoculating their hands or ice scoops with E. coli and then handling the two items.
Their idea is to expand the range of the truffle in North America using a proprietary new technique, developed by Dr. Thomas, for inoculating the roots of trees to more reliably grow the fungus.
He's inoculating himself, and no price — not the reputations of individuals who have behaved honorably, not the viability of institutions that are crucial to the health of our democracy — is too steep to pay.
In reality, instead of inoculating a piece against judgment, this posture creates a kind of critical stalemate: If you already know what your readers are going to say, why should we bother saying it?
The language in the contract seemed "aimed at inoculating a company against future claims" of sexual harassment, according to several lawyers interviewed by the Boston Globe, with at least one suggesting it was legally unenforceable.
By making the CFPB director difficult to fire and inoculating its budget from the whims of congress, the architects, which include the likes of Elizabeth Warren, designed a rare bureaucracy that can act efficiently and aggressively.
Inoculating the Southern Hemisphere from the growing scourge of sex abuse scandals, spreading secularism and out-of-touch clergy that devastated Catholicism in Europe is no easy task and will require much of the pope's attention.
Right now, for example, health workers are pursuing a ring vaccination strategy, which requires tracking down every person that an Ebola patient came into contact with when they were ill, and inoculating them to stop its spread.
Related: Muslim Civil Rights Group Files Class Action Lawsuit Challenging Terror Watch List By filing the lawsuit, Microsoft could be inoculating itself against claims that it is complicit in letting the government eavesdrop on people's cloud accounts.
Inoculating yourself from lies These efforts are often called "inoculations," since they use a real-life example in one domain to teach people about the strategies used to spread lies and therefore equipping people to spot them more easily.
By surrounding the last places on earth where smallpox was still occurring — small villages in Asia and Africa — and inoculating everyone in a wide circle around them, Henderson and the World Health Organization were able to starve the virus of hosts.
Even though the virus had spread from a rural area to a thriving lakeside town and ultimately to a big city, Mbandaka, the outbreak was quickly stopped by inoculating health workers and the rings of contacts of each known case.
Pete Buttigieg worked nearly three years for the consulting firm McKinsey & Company, and he has presented that experience as a kind of capitalist credential — distinguishing him from some rivals for the Democratic presidential nomination, and inoculating him against Republican attacks.
It is an era that should be ripe for journalists and for the business of journalism — a profession that, though it errs often, is the best way we know of inoculating ourselves against the suffocating deluge of rumor and mendacity.
The plan right now is to start with a ring vaccination approach, which involves inoculating the contacts of people who are infected with Ebola (as well as health professionals and other responders), effectively creating a "ring" of protection around them.
For although it was Lady Mary Wortley Montagu who first introduced the inoculation procedure to Western medicine, it was Caroline who, in the new spirit of scientific empiricism, arranged experiments, and who, by inoculating her own children, spread the practice more widely.
On Monday, a team led by the World Health Organization began inoculating people in the Democratic Republic of Congo against the Ebola virus with an experimental vaccine in an attempt to quell an outbreak of the disease that began in early May.
LOT works by having member companies sign an agreement wherein if any member's patents fall into the hands of a PAE, the other members of LOT automatically get a license to those patents — inoculating LOT members from getting sued for infringement using those patents.
In a 75-page illustrated manual, Jenner explained how people could protect themselves from smallpox – a horrific brute of a disease that killed one person in 12 and left many survivors scarred for life – by inoculating themselves with cowpox, an obscure disease that affected cattle.
Violence may be prevented, then, by interfering with this process in any of several ways: hindering the speech, restricting its dissemination, undermining the credibility and integrity of the speaker, or "inoculating" the audience against the speech so that it is less influential, or dangerous.
For founders, the biggest challenge is inoculating their teams from the vagaries of the market so they can do their jobs, continue building momentum against this market adversity and, ultimately, ensure there is enough cash in the bank to avoid layoffs and sustain their company for growth.
The results demonstrate that a campaign led by transgender activists, relying on young transgender people to become the face of their efforts, prevailed at inoculating voters against unfounded smears about "men in women's bathrooms" — a line of attack used by the Christian right elsewhere to reverse protections for transgender people.
Inoculating against misinformation The researchers characterize their strategy to mitigate the potency of misinformation to influence opinion as akin to a medical inoculation, whereby a small amount of a virus can be used to bolster immune resistance to an illness ahead of time, before a person encounters the virus in the wild.
She could dabble in fringe views—she may even have a history of dubious tweets the elites send around to scoff at—but her pre-politics status as a mass pop cultural figure has the odd benefit of inoculating her against the political media's attempts to define her as outside the mainstream.
To that end, Ms. Warren, an Oklahoma native, used a recounting of her roots to not just tell her family's ethnic story but to present a tale of Dust Bowl hardship that has the makings of a stump speech aimed at inoculating her against charges of being a member of the coastal elite.
He has disarmed it of its terrour at Muack, by inoculating eighty of his people.
Inoculating mosquitoes with this strain reduced the prevalence of the Malaria causing organism (Plasmodium) by up to 98%.
Success has been achieved in germinating seeds of P. arenicola in vitro by inoculating them with a mychorrizal fungus.
Dr. morbific, by inoculating himself once too often with non-contagious matter, had explained himself out of the world.
His aim was to enlighten the peasants by example. Chydenius practiced medicine and became known by inoculating ordinary people against smallpox. He also performed cataract operations and prepared medicines. Statue of Anders Chydenius in Kokkola, Finland.
Gose is a top-fermenting beer that originated in Goslar, Germany. This style is characterized by the use of coriander and salt and is made sour by inoculating the wort with lactic acid bacteria before primary fermentation.
Basal stem rot (BSR), caused by the fungus Ganoderma, is the most serious disease of oil palm in Malaysia and Indonesia. Previously, research on basal stem rot was hampered by the failure to artificially infect oil palms with the fungus. Although Ganoderma had been associated with BSR, proof of its pathogenicity to satisfy Koch's postulate was only achieved in the early 1990s by inoculating oil palm seedling roots or by using rubber wood blocks. A reliable and quick technique was developed for testing the pathogenicity of the fungus by inoculating oil palm germinated seeds.
Screening for VRE can be accomplished in a number of ways. For inoculating peri-rectal/anal swabs or stool specimens directly, one method uses bile esculin azide agar plates containing 6 μg/ml of vancomycin. Black colonies should be identified as an enterococcus to species level and further confirmed as vancomycin resistant by an MIC method before reporting as VRE. Vancomycin resistance can be determined for enterococcal colonies available in pure culture by inoculating a suspension of the organism onto a commercially available brain heart infusion agar (BHIA) plate containing 6 μg/ml vancomycin.
The Sereny test is a test used to test the invasiveness of enteroinvasive Escherichia coli, Shigella species, and Listeria monocytogenes. It is done by inoculating suspension of bacteria into guinea pig's eye. Severe mucopurulent conjunctivitis and severe keratitis indicates a positive test.
Inoculate Simmons citrate agar (do not inoculate heavily) by using straight wire from an 18- to 24-hour old colony. Inoculating from a broth culture is not recommended because the inoculum would be too heavy. Incubate at 35 °C for up to seven days.
Australia, Brazil and the United States are currently suffering from canker outbreaks. The disease can be detected in orchards and on fruit by the appearance of lesions. Early detection is critical in quarantine situations. Bacteria are tested for pathogenicity by inoculating multiple citrus species with the bacterium.
Motility is determined by using a motility medium. The ingredients include motility test medium, nutrient broth powder, NaCl and distilled water. An inoculating needle (not a loop) is used to insert the bacterial sample. The needle is inserted through the medium for a length of one inch.
Alternatively, insect cells can be grown at room temperature. Most bioreactors are maintained at 5% carbon dioxide. Cells can either be cultivated in continuous or fed-batch systems. The former entails inoculating and harvesting cells in a constant process so that there are always cells in the bioreactor.
Augustine: Ancient Thought Baptized (Cambridge University Press, 1994). Italian edition (Milan: Vita e Pensiero, 1997); Spanish (Ediciones Destino) edition planned. 11\. Man, Soul and Body: Essays in Ancient Thought from Plato to Dionysius (Ashgate Variorum: London, 1996). 12\. On Inoculating Moral Philosophy against God (Marquette University Press, 2000). 13\.
However, Jesty simply performed the procedure; he did not take the discovery any further by inoculating his family with smallpox matter to see if there would be a reaction or perform any other trials. Jenner was the first to prove the effectiveness of vaccination with cowpox using scientific experimentation.
Sara Branham inoculating antiserum into a mouse to determine whether it would protect against meningitis, Robert Forkish assisting, 1937 left left Sara Elizabeth Branham Matthews (1888–1962) was an American microbiologist and physician best known for her research into the isolation and treatment of Neisseria meningitidis, a causative organism of meningitis.
Jellison, pp. 10–11 He was also called to court in Salisbury for inoculating himself against smallpox, a procedure that required the sanction of the town selectmen.Jellison, p. 12 Allen met Thomas Young when he moved to Salisbury, a doctor living and practicing just across the provincial boundary in New York.
According to Voltaire (1742), the Turks derived their use of inoculation from neighbouring Circassia. Voltaire does not speculate on where the Circassians derived their technique from, though he reports that the Chinese have practiced it "these hundred years". In 1721, Cotton Mather and colleagues provoked controversy in Boston by inoculating hundreds.
The murderer does strike again, but Brad had taken the precaution of inoculating the jockey beforehand. Brad invites all of the suspects to his suite, hoping the culprit will stay away. When that fails, he screens the film footage he had taken. It shows Mr. Summers slipping a capsule down the jockey's back.
Kefir or kephir ( ),"kefir". Oxford Dictionaries.kefir. dictionary.reference.com is a fermented milk drink similar to a thin yogurt that is made from kefir grains, a specific type of mesophilic symbiotic culture. The drink originated in the North Caucasus, Eastern Europe and Russia, where it is prepared by inoculating cow, goat, or sheep milk with kefir grains.
A scheme for inoculating poor people for smallpox was started in 1784. They were attended, when necessary, in their own homes, as were people with contagious diseases. Jenner's scheme of vaccination was enthusiastically adopted in 1800, and 1,000 people were vaccinated in six months. In 1790 two physicians were appointed to deal with home visits.
The knowledge of inoculating oneself against smallpox seems to have been known to West Africans, more specifically the Akan people. A slave named Onesimus explained the inoculation procedure to Cotton Mather during the 18th century; he reported to have acquired the knowledge from Africa.Waldstreicher, David. Runaway America: Benjamin Franklin, Slavery, and the American Revolution.
On 27 April 1943 Lush accidentally pricked her finger with a needle containing scrub typhus while inoculating a mouse. There was no effective treatment at the time for this often fatal disease. She died four weeks later, on 20 May 1943. Before her death she insisted that blood samples be taken from her to aid research.
He described to the Committee for the Regulation of the Police the appalling living conditions in cellars without lighting, sanitation or ventilation. People newly arrived from the country were particularly vulnerable to fevers. The Board pressed for the wheels of machinery to be guarded against accidents. A scheme for inoculating poor people for smallpox was started in 1784.
Routine laboratory tests may be normal because the organism is difficult to culture. Inoculating the fluid from infected joints directly into blood culture vials can enhance the chances of an accurate culture, but extended culture times are not helpful. The organism has also been known as Moraxella kingae. K. kingae is oxidase-positive, catalase-negative, and beta- hemolytic.
Halophilic extremophiles live in areas of high salinity such as solar salterns and soda lakes. Their ability to consume and thrive in areas of such salinity open up possible benefits such as inoculating crops in salt rich soils to help them grow. Another use found for them lies in their production of polymers used to make biodegradable plastics.
The exact origin of the modern smallpox vaccine is unclear. Edward Jenner had obtained his vaccine from the cow, so he named the virus vaccinia, after the Latin word for cow. Jenner believed that both cowpox and smallpox were viruses that originated in the horse and passed to the cow. Some doctors followed up on this speculation by inoculating humans with horsepox.
Alexis is hospitalized after being lured into a trap by Jerry Jacks, her old flame. He later reveals that he was actually inoculating her against a pathogen that he put in the Port Charles water supply, infecting the whole town. He demands a large sum of money in exchange for the antidote. Jerry kidnaps her, hoping to disappear with Alexis and the money.
The microbe, or the inoculating agent, must come from the same colony-forming unit, and must be at the correct concentration. This may be adjusted by incubation time and dilution. For verification, the positive control is plated in a hundred fold dilution to count colony forming units. The microbes inoculate the tubes (or plate) and are incubated for 16–20 hours.
A bacterial culture was obtained by inoculating fertilized chicken eggs with C. felis. After the strain had gone through several chicken egg passages, the strain went through four passages of McCoy cells before finally being used for genetic analysis. The genome of C. felis was sequenced via whole genome shotgun. Each gene was then annotated by programs BLASTP and FASTA.
CTV is classically diagnosed by graft- inoculating a Mexican lime (Citrus aurantifolia) with tissue from a diseased plant. The Mexican lime will develop highly predictable symptoms. Symptoms on the leaves begin as clear veins that turn corky, which is then followed by chlorosis and cupping of the leaf. Particularly severe strains may result in stunting, and stem-pitting may also occur.
Short-term culturing of E. bieneusi was achieved by inoculating duodenal aspirate and biopsy specimens into E6 and HLF monolayers. The short-term cultures lasted up to 6 months. After several weeks of culture, gram-positive spore-like structures measuring 1 to 1.2 um long were observed. Mature spores and sporoblasts with double rows of polar tubule coils were seen (Visvesvara 2002).
Plant manufactured Pharmaceuticals are pharmaceuticals derived from genetically modified plants used as therapeutic compounds. This can be used as the replacement for the traditional method of inoculating animals for Cell Culture production. We can use plants to cure and prevent diseases that may have once been deemed incurable. Through biotechnological advancements, we are able to produce complex therapeutic proteins from plant cells.
Microbial aerosols can be created from the hitting of the inoculation tip to the sides of the agar plate. The inoculation needle is then withdrawn from the inoculated agar plate culture and flamed. ; Stab culture An inoculation needle is an essential tool in inoculating a stab culture. A sterile stab culture cap is removed and the open end is flamed.
With proactive programs of inoculating local cattle, the population of Gaur is again on the rise. Another concern due to closeness of the population is procurement of non-timber forest products for commercial purposes and the procurement of timber for firewood. These affect the health of the forest in a long run. The other large threats are fishing and illegal poaching of wild animals.
The disease can be detected in groves and on fruit by the appearance of lesions. Early detection is critical in quarantine situations. Bacteria can be tested for pathogenicity by inoculating multiple citrus species with them. Additional diagnostic tests (antibody detection), fatty- acid profiling, and genetic procedures using polymerase chain reaction can be conducted to confirm diagnosis and may help to identify the particular canker strain.
The earliest account of mathematical modelling of spread of disease was carried out in 1760 by Daniel Bernoulli. Trained as a physician, Bernoulli created a mathematical model to defend the practice of inoculating against smallpox. The calculations from this model showed that universal inoculation against smallpox would increase the life expectancy from 26 years 7 months to 29 years 9 months. Daniel Bernoulli's work preceded the modern understanding of germ theory.
An antibody digested by pepsin yields two fragments: a F(ab')2 fragment and a pFc' fragment. Redback spider antivenom contains purified F(ab')2 derived from IgG in hyperimmune horse plasma. Redback antivenom was developed by Commonwealth Serum Laboratories, then a government body involved with discovering antivenoms for many venomous Australian creatures. Production involves the milking of venom from redbacks and repeatedly inoculating horses with non-lethal doses.
After Hunter's experiment other scientists sought to disprove his conclusions by inoculating other male physicians, medical students, and incarcerated men with gonorrheal pus, who all developed the burning and discharge of gonorrhea. One researcher, Ricord, took the initiative to perform 667 inoculations of gonorrheal pus on patients of a mental hospital, with zero cases of syphilis. Notably, the advent of penicillin in the 1940s made effective treatments for gonorrhea available.
Dr. Sze became chief of specialized agencies for the Eco-nomic & Social Council of the United Nations. He was greatly disappointed when he was later offered a job at WHO that he couldn't take because of his U.N. commitments. He became U.N. medical director in 1954, taking care of the permanent staff of about 3000, including inoculating them and preparing them for missions abroad. He held the position for 20 years.
Known as a “tinkerer” among friends, Hingson developed several important medical devices such as the Western Reserve Anesthesia Machine, Oxygen Inhalator, and Resuscitator. With the help of colleague Waldo B. Edwards, he also developed the continuous caudal anesthesia epidural administration to alleviate the pain of childbirth. In 1938, Hingson developed a painless method of inoculating patients. Known as the jet injector this was perhaps his greatest contribution to medicine.
In 1928, Frederick Griffith demonstrated transformation of life turning harmless pneumococcus into a lethal form by co-inoculating the live pneumococci into a mouse along with heat-killed virulent pneumococci. In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty demonstrated that the transforming factor in Griffith's experiment was not protein, as was widely believed at the time, but DNA. Avery's work marked the birth of the molecular era of genetics.
In 1914, Michael Levine was the first to report successfully cultivating C. micaceus from spores in the laboratory. In his experiments, fruit bodies appeared roughly 40 to 60 days after initially inoculating the growth media (agar supplemented with soil, horse dung, or cornmeal) with spores. Like other coprinoid species, C. micaceus undergoes synchronous meiosis. The chromosomes are readily discernible with light microscopy, and all of the meiotic stages are well-defined.
During their research, they found that the levels of these cross-reactive T cells are significantly elevated in multiple sclerosis patients. thumb Some research has shown that inoculating an animal with MBP to generate an MBP-specific immune response against it increases blood–brain barrier permeability. Permeability is enhanced when the animal is inoculated against non-specific proteins. A targeted immune response to MBP has been implicated in lethal rabies infection.
The Global Warming Petition Project, also known as the Oregon Petition, is a petition urging the United States government to reject the global warming Kyoto Protocol of 1997 and similar policies. Some consider it to be a political petition designed for disinforming and confusing the public about the scientific results and the consensus of climate change research.Sander van der Linden et al.: Inoculating the Public against Misinformation about Climate Change.
PF Tek is a popular method for home cultivation because it is easy, simple, inexpensive and reliable. The materials needed are cheap and widely available. Jars can be sterilized by boiling or with a pressure cooker. Although sterile technique is required when inoculating, risk of contamination from airborne spores or bacteria is small because air exposure is minimal and the layer of vermiculite which acts as a filter.
Since this is true, higher amounts of distinctive pathogenic nucleic acid sequences would be in those exhibiting disease compared to controls since inoculating those without the pathogen is unethical. In addition, the DNA load should drop or become lower with the resolution of the disease. The distinctive pathogenic nucleic acid sequences load should also increase upon recurrence. Other conditions are met to establish cause or association including studies in disease transmission.
Haffkine's vaccine saved thousands of lives with Viegas personally inoculating around eighteen thousand residents. After his death in 1933, a life-size statue of him was erected in the Cowasji Jehangir Hall opposite Metro Cinema on his birth centenary in 1956, by the Governor of Bombay Presidency, Harekrushna Mahtab, as a tribute to the services rendered to the city. A street in the Dhobitalao area is also named after him.
In a 1987 study completed by Postlethwait et al., researchers assessed the immune response of the medfly using bacterial inoculation. After inoculating the medfly with Enerobacter cloacae, the researcher extracted the haemolymph from the males and found that it contained potent antibacterial factors compared to the haemolymph of controls. Through further testing, they were able to show that these potent factors were generated within 3 hours of inoculation and lasted for approximately 8 days.
Denitrification also produces nitrous oxide (N2O), which is a greenhouse gas that is detrimental to the environment. Production of N2O contributes to the loss of fixed nitrogen, which regulates the biological productivity of ecosystems. By inoculating wastewater reactors with the anaerobic S. wagneri, operation costs can be reduced by about ninety percent without the production of greenhouse gases. This allows for better wastewater management in a more cost-efficient manner without contributing to climate change.
Ceratocystis paradoxa or Black Rot of Pineapple is a plant pathogen that is a fungus part of the phylum Ascomycota. It is characterized as the teleomorph or sexual reproduction stage of infection. This stage contains ascocarps, or sacs/fruiting bodies, which contain the sexually produced inoculating ascospores. These are the structures which are used primarily to survive long periods of time or overwinter to prepare for the next growing season of its host.
Frith, Hollander, Jauniaux) – 4:58 #"Palmiers en Pots" ##"[Trio (from Nuits D'Argentine)]" (Verchuren) – 1:25 ##"[Tango]" (Wuyts, Hollander) – 1:59 #"Geistige Nacht [Rondo]" (Frith, arr. Frith) – 5:18 #"I Viaggi Formano la Gioventú [Truc Turc]" (trad. Turkish folk song arr. Kenis, Aksak Maboul) – 5:09 #"Inoculating Rabies [Pogo]" (van Hecke, Wuyts) – 1:47 ;Side B #"Cinema [Knokke]" ##"Ce Qu'On Peut Voir Avec Un Bon Microscope" (Hollander, Wuyts, Aksak Maboul) – 7:25 ##"Alluvions" (Hollander, Wuyts, arr.
The Hawley Society based in large part on The Hawley Record, by Elias Sill Hawley, 1890 The Boylstons were one of the most respectable families in New England and among her relatives was cousin Ward Nicholas Boylston, a benefactor of Harvard College, and uncle Zabdiel Boylston, the celebrated Physician who performed the first surgical operation by an American physician and was known for inoculating hundreds of people in Boston during a severe smallpox outbreak.
In 1796, Edward Jenner, a doctor in Berkeley, Gloucestershire, rural England, discovered that immunity to smallpox could be produced by inoculating a person with material from a cowpox lesion. Cowpox is a poxvirus in the same family as variola. Jenner called the material used for inoculation vaccine from the root word vacca, which is Latin for cow. The procedure was much safer than variolation and did not involve a risk of smallpox transmission.
Methods of carrying out the procedure varied depending upon location. Variolation was the sole method of protection against smallpox other than quarantine until Jenner's discovery of the inoculating abilities of cowpox against the smallpox virus in 1796. Efforts to protect populations against smallpox by way of vaccination followed for centuries after Jenner's discovery. Smallpox has since been completely eradicated since 1979, because of the mass vaccination efforts of the World Health Organization.
New York: Cambridge University Press, 2011 where 800 people were inoculated and only eight deaths occurred. His account of the success of variolation in Charleston helped to play a role in the revival of its practice in London. Kirkpatrick also advocated inoculating patients with matter from the sores of another inoculated person, instead of using matter from the sore of a smallpox victim, a procedure that Maitland had been using since 1722.
In 1753, Alexander resigned, returning to London and publishing a Natural History of Aleppo and Parts Adjacent in 1756. Patrick took up the position left by Alexander and worked for about 18 years. The Pasha of Aleppo held him in high regard, even honouring him with a turban. A keen observer of traditions, he noted in a letter read by Alexander to the Royal Society an Arabian practice of inoculating children against smallpox using "variolus matter".
William Woodville with 'View of the inoculating Hospital at Pancras' (1806). William Woodville was born at Cockermouth in Cumberland in 1752 in wealthy Quaker family. He apprenticed to the apothecary William Birtwhistle before going to study medicine at Edinburgh University, where he became the favourite pupil of William Cullen, and graduated M.D. on 12 September 1775. After spending some time on the continent he began to practise at Papcastle in his native county, but shortly afterwards moved to Denbigh.
"Floods in Rumania Peril Cities' Dikes", The New York Times, May 27, 1970, p.9 Significant resources were mobilised to fight the waters and their effects. Medical teams toured most areas of the country warning people not to drink polluted water (many drinking water installations were destroyed) and inoculating all citizens aged 2 to 55 against typhoid fever. Thousands of soldiers and civilians worked around the clock to build miles of new dikes and reinforce old ones.
The adult chicken has also made significant contributions to the advancement of science. By inoculating chickens with cholera bacteria (Pasteurella multocida) from an overgrown, and thereby attenuated, culture Louis Pasteur produced the first lab-derived attenuated vaccine (1860s). Great advances in immunology and oncology continued to characterize the 20th century, for which we indebted to the chicken model. Peyton Rous (1879-1970) won the Nobel prize for discovering that viral infection of chicken could induce sarcoma (Rous, 1911).
Villemin was born in the department of Vosges, and studied medicine at the military medical school at Strasbourg, qualifying as an army doctor in 1853. Afterwards he practiced medicine at the military hospital of Val-de-Grâce in Paris. In 1874 he became a member of the French Académie Nationale de Médecine, and was its vice-president in 1891. Villemin proved that tuberculosis was an infectious disease by inoculating laboratory rabbits with material from infected humans and cattle.
Scott's method involved inoculating blood into two rubber-sealed glass bottles, one for aerobes and one for anaerobes. The aerobic bottle contained trypticase soy broth and an agar slant, and the anaerobic bottle contained thioglycollate broth. The lysis-centrifugation method was introduced in 1917 by Mildred Clough, but it was rarely used in clinical practice until commercial systems were developed in the mid-1970s.TeKippe, EM & Pence, MA. Chapter 3 in Dunne, WM & Burnham, CAD eds. (2018). sec.
The spread likely is by spores rather than roots. The knowledge gained can be employed in the rapid degradation of oil palm waste on the plantation floor by inoculating suitable fungi, and/or treating the waste more appropriately (e.g. chipping and spreading over the floor rather than windrowing). Endophytic bacteria are organisms inhabiting plant organs that at some time in their life cycles can colonize the internal plant tissues without causing apparent harm to the host.
Symptoms of panicum mosaic virus on millet—without its satellite virus—are slight chlorosis and mild stunting. The synergistic effect of panicum mosaic virus and satellite panicum mosaic virus on millet cultivars is rapidly developed chlorotic streaking within several days of inoculating plants. The long-term effects of combined panicum mosaic and satellite panicum mosaic viruses on millet are severe leaf mosaicking, stunting, and failure to set seed.Qi, D., Omarov, R. T., & Scholthof, K. B. G. (2008).
He ordered his men to aim for the British officers, knowing the crippling effect it would have. He knew the value of inoculating the American troops against small pox, and the tendency of nervous soldiers to fire too soon and aim too high (thus the orders to not fire until "you see the whites of their eyes" and to "Take aim at the waistbands.").Thompson, John M. The Revolutionary War, p. 38, National Geographic Society, Washington, D.C., 2004. .
A small number of asymptomatic carriers of polio (referred to as chronic excretors) continue to produce active virus for years (or even decades) after their initial exposure to the oral Sabin vaccine. Carriers of the attenuated virus unintentionally spread the attenuated virus, inoculating others, giving them contact immunity; however some adults with weak immune systems have contracted paralytic polio from contact with recently immunized children. Carriers of virulent strains spread polio, increasing the difficulty of poliomyelitis eradication.
In inoculation the inoculation needle is first employed to transfer microbial life forms from a culture to the needle to be used in further inoculating procedures. Sources of inoculum include broth cultures, slant cultures, and plate cultures. ; Broth culture An inoculation needle is used in the transfer of microorganisms from a broth culture by first employing a vortex mixer to ensure a uniform suspension of the microorganisms within the broth. Aseptic technique is then applied to the needle.
Stab Cultures after inoculation by inoculation needle with E. coli from left to right: TSI, Soft nutrient, Simmons Citrate agar Inoculation of the microorganisms will be done directly after the transfer from culture to inoculation needle. The inoculum is commonly inoculated to broth cultures, slant cultures, plate cultures, and stab cultures. ; Broth culture An inoculation needle is used in inoculating a sterile broth culture. Flaming the open end of the broth will keep the it sterile.
So-called blue cheese is created by inoculating a cheese with Penicillium roqueforti or Penicillium glaucum. This is done while the cheese is still in the form of loosely pressed curds, and may be further enhanced by piercing a ripening block of cheese with skewers in an atmosphere in which the mold is prevalent. The mold grows within the cheese as it ages. These cheeses have distinct blue veins, which gives them their name and, often, assertive flavors.
It soon began to spread to humans and be transmitted by the common Aedes aegypti mosquitoes. Blood specimens were taken from over 4,500 humans in late 1953 and early 1954, and checked to detect the presence of a wide variety of known viruses. Over 15% showed antibodies to yellow fever and more human cases quickly followed. Warnings were made that an epidemic was imminent and Downs and Hill began a program of inoculating health workers and stockpiling vaccine.
A lesion on the hand of a person with cat-scratch disease. Cat-scratch disease commonly presents as tender, swollen lymph nodes near the site of the inoculating bite or scratch or on the neck, and is usually limited to one side. This condition is referred to as regional lymphadenopathy and occurs 1–3 weeks after inoculation. Lymphadenopathy in CSD most commonly occurs in the arms, neck, or jaw, but may also occur near the groin or around the ear.
The packaged inoculant could therefore be used successfully only in plants of the particular group corresponding to this bacterial species. Thus, if a farmer had crops of clover, alfalfa, and soybeans, he would have to use three separate inoculants. The patentee discovered that there are strains of each species of root nodule bacteria which do not exert a mutually inhibitive effect on each other. Thus, he was able to provide a mixed culture of Rhizobia capable of inoculating plants belonging to several groups.
Testing is performed by an audiologist. There is no proven or recommended treatment or cure for SNHL; management of hearing loss is usually by hearing strategies and hearing aids. In cases of profound or total deafness, a cochlear implant is a specialised hearing aid that may restore a functional level of hearing. SNHL is at least partially preventable by avoiding environmental noise, ototoxic chemicals and drugs, and head trauma, and treating or inoculating against certain triggering diseases and conditions like meningitis.
Fed-batch systems mean inoculating the cells, culturing them and harvesting them in one distinct period. Stirred tank bioreactors are the most widely used configuration in which an impeller increases the flow, thereby homogenizing the culture media and a diffuser facilitates the exchange of oxygen into the media. This system is generally used for suspended cultures but can also be used for cells which require attachment to another surface if microcarriers are also included. Fixed bed bioreactors are commonly used for adherent cultures.
Historically, chiropractic strongly opposed vaccination based on its belief that all diseases were traceable to causes in the spine and therefore could not be affected by vaccines. Daniel D. Palmer (1845–1913), the founder of chiropractic, wrote: "It is the very height of absurdity to strive to 'protect' any person from smallpox or any other malady by inoculating them with a filthy animal poison." Vaccination remains controversial within the profession. Most chiropractic writings on vaccination focus on its negative aspects.
Louis Wendlin Sauer (b. August 13, 1885 in Cincinnati; d. February 10, 1980 in Miami)"Cough vaccine inventor dies", Santa Rosa (CA) Press Democrat, February 11, 1980, p1 was an American pediatrician who became known for perfecting the vaccine used to prevent pertussis (whooping cough), saving countless lives around the world. After five years of work, Dr. Sauer developed the vaccine in 1931, inoculating children against pertussis, a respiratory infection that had been the most fatal disease for children under two years old.
Cotton Mather promoted America's first public inoculation campaign. Cotton Mather sent letters to Boston's 14 other physicians in response to the outbreak imploring them to wage a medical campaign against smallpox by inoculating their own patients or volunteers. Mather had been interested in inoculation since 1715 when his slave, Onesimus, informed Mather about a procedure in Africa which made him immune to smallpox for life. Mather read physician Emmanuel Timoni's description of a similar procedure witnessed while serving Great Britain's ambassador in Turkey.
The chronic phase causes digestive problems, constipation, heart failure, and pain in the abdomen. Diagnostic methods include microscopic examination, serology, or the isolation of the parasite by inoculating blood into a guinea pig, mouse, or rat. No vaccines are available. The most used method for epidemiological management and disease prevention resides within vector control, mainly by the use of insecticides, specially pyrethroids, and taking preventative measures such as applying bug repellent on the skin, wearing protective clothing, and staying in higher quality hotels when traveling.
The first step is to prepare the cultures by inoculating milk with 2–3% grains as described. The grains are then removed by filtration and 1–3% of the resulting liquid mother culture is added to milk and fermented for 12 to 18 hours. Kefir can be made using freeze-dried cultures commonly available in powder form from health food stores. A portion of the resulting kefir can be saved to be used a number of times to propagate further fermentations but ultimately does not form grains.
Another possibility for coral restoration is gene therapy: inoculating coral with genetically modified bacteria, or naturally-occurring heat-tolerant varieties of coral symbiotes, may make it possible to grow corals that are more resistant to climate change and other threats. Warming oceans are forcing corals to adapt to unprecedented temperatures. Those that do not have a tolerance for the elevated temperatures experience coral bleaching and eventually mortality. There is already research that looks to create genetically modified corals that can withstand a warming ocean.
Typically, basidiospores infect host one, also known as the alternate or sexual host, and the mycelium forms pycnidia, which are miniature, flask-shaped, hollow, submicroscopic bodies embedded in the host tissue (such as a leaf). This stage, numbered "0", produces single-celled spores that ooze out in a sweet liquid and that act as nonmotile spermatia, and also protruding receptive hyphae. Insects and probably other vectors such as rain carry the spermatia from spermagonium to spermagonium, cross inoculating the mating types. Neither thallus is male or female.
The fungicide does not kill the pathogen itself, but works on the pre-necrotic spots on the leaves, stopping the secondary spores from inoculating new, healthy plant tissue. The best time to apply this protective fungicide is in the beginning of the season in order to stop any initial infection. The class of fungicides widely used to control black leaf streak is the triazoles. These are demethylation inhibitors and should be rotated with compounds having other modes of action to slow the development of resistance.
Diacetyl arises naturally as a byproduct of fermentation. In some fermentative bacteria, it is formed via the thiamine pyrophosphate-mediated condensation of pyruvate and acetyl CoA. Sour (cultured) cream, cultured buttermilk, and cultured butter are produced by inoculating pasteurized cream or milk with a lactic starter culture, churning (agitating) and holding the milk until a desired pH drop (or increase in acidity) is attained. Cultured cream, cultured butter, and cultured buttermilk owe their tart flavour to lactic acid bacteria and their buttery aroma and taste to diacetyl.
In 1953 researchers found that laboratory animals could be helped not to reject organ transplants by pre-inoculating them with cells from donor animals; in 1968, in Minnesota, the first successful human bone marrow transplantation took place. In more recent work, cell encapsulation is pursued as a means to shield therapeutic cells from the host immune response. Recent work includes micro-encapsulating cells in a gel core surrounded by a solid, but permeable, shell. Bone marrow transplants are the most common and well established cell transplantation therapies.
Studies investigating the transmission of the disease have found that it can be transmitted to susceptible deer by inoculating them with infected material from diseased deer by subcutaneous, intramuscular, intravenous, or oral routes in experimental settings. The specific tissues and organs that show hemorrhaging from EHDV vary animal to animal. The most common ones include the heart, liver, spleen, kidney, lung, and intestinal tract. Virus samples can be isolated from many kinds of tissues from infected animals, including blood, liver, spleen, kidney, lung, heart, and other muscles.
Minimum inhibitory concentrations of penicillins, rifampicin, and azithromycin are easily achieved. Multiple studies have shown that inoculating synovial fluid or bone samples directly into blood culture bottles substantially increases the detection of K. kingae compared with direct plating of specimens on solid media. As an oropharyngeal colonizer, K. kingae is transmitted by respiratory secretions, saliva, and potentially oral contact with contaminated objects. Recent studies suggest K. kingae strains may demonstrate varying degrees of pathogenicity, which could support the person-to-person transfer of pathogenic K. kingae.
She earned her medical degree at the Woman's Medical College of Pennsylvania (now part of the Drexel University College of Medicine) and performed her internship and residency at Philadelphia General Hospital, where she met her future husband, the physician Eric Reiss. The couple first moved to San Antonio, Texas, then relocated to St. Louis after Eric Reiss received an appointment at the Washington University School of Medicine. Hired by the St. Louis city health department, Louise Reiss was involved in inoculating children with the polio vaccine.
Until this point, researchers debated whether syphilis and gonorrhea were manifestations of the same disease or two distinct entities. One such 18th-century researcher, John Hunter, tried to settle the debate in 1767 by inoculating a man with pus taken from a patient with gonorrhea. He erroneously concluded that both syphilis and gonorrhea were indeed the same disease when the man developed the copper- colored rash that is classic for syphilis. Although many sources repeat that Hunter inoculated himself, others have argued that it was in fact another man.
The first camembert was made from unpasteurized milk, and the AOC variety "Camembert de Normandie" (approximately 10% of the production) is required by law to be made only with unpasteurized milk. Many modern cheesemakers, however, use pasteurized milk for reasons of safety, compliance with regulations, or convenience. True made with unpasteurized milk The cheese is made by inoculating warmed cow milk with mesophilic bacteria, then adding rennet and allowing the mixture to coagulate. The curd is then cut into roughly 1 cm (1/2 inch) cubes, salted, and transferred to low cylindrical camembert molds.
St. Mary's Church and churchyard Edward Jenner, the originator of vaccination, was born in Berkeley. After studying medicine in London he returned home to work as the local doctor, and in 1796, realising that milkmaids didn't catch smallpox, he performed a pioneering experiment by inoculating his gardener's son James Phipps with cowpox, thus preventing infection from smallpox. Jenner is buried in the family vault at the Church of St Mary, Berkeley; Phipps is also buried there. The Chantry, Jenner's home in Berkeley for 38 years, is now a museum.
The most common method to isolate individual cells and produce a pure culture is to prepare a streak plate. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate. Upon incubation, colonies will arise and single cells will have been isolated from the biomass. Once a microorganism has been isolated in pure culture, it is necessary to preserve it in a viable state for further study and use.
Since Wolbachia is also maternally transmitted, it was found that the WNV-resistant phenotype is directly related to the Wolbachia infection. West Nile virus is transmitted to humans and animals through the Southern house mosquito, Culex quinquefasciatus. Glaser and Meola knew vector compatibility could be reduced through Wolbachia infection due to studies done with other species of mosquitoes, mainly, Aedes aegypti. Their goal was to transfer WNV resistance to Cx. quinquefasciatus by inoculating the embryos of the mosquito with the same strain of Wolbachia that naturally occurred in the fruit flies.
Rajat ("Raj") Parr is an Indian-American sommelier turned winemaker, who oversaw the wine program of the Michael Mina restaurant group before starting his own wineries in Oregon and the Central Coast of California. Sandhi is his label of purchased grapes while Lompoc, Domaine de la Cote, and Evening Land all are productions from his and Sashi Moorman's (his business partner) vineyards. He produces Pinot Noir and Chardonnay mainly, biodynamically and organically. His winemaking philosophy is based on little to no manipulation of the final product by never inoculating nor over-ripening.
After working in the rabies division of Rue Vaquelin and studying the microbe that causes dysentery, André Chantemesse collaborated with a younger bacteriologist, Georges-Fernand Widal. Together they were able to immunize guinea pigs by inoculating them with heat-treated dead bacteria, calling into question the notion that only weakened, not dead, bacteria can be used to immunize. They concluded that a series of three or four early injections of such heat-inactivated bacteria can effectively inoculate against development of the disease, as the endotoxins alone are sufficient to trigger the production of antibodies.
002 The anaerobic roll-tube technique was utilized in isolation of anaerobic bacteria by inoculating them into anoxygenic agar tubes.Lillian V. Holdeman, W. E. C. Moore; Roll-tube techniques for anaerobic bacteria, The American Journal of Clinical Nutrition, Volume 25, Issue 12, 1 December 1972, Pages 1314–1317 The media in the tubes was Peptone Yeast Agar containing trypticase, yeast extract, Na2CO3, L-cysteine hydrochloride dihydrate and sodium resazurin. Strain WK011T was then sequenced and found to have a 95% genome similarity to both Z. raffinosivorans and Propionispira arboris.
The knowledge of inoculating oneself against smallpox seems to have been known to West Africans, more specifically the Akan. A slave named Onesimus explained the inoculation procedure to Cotton Mather during the 18th century; he reported to have gotten the knowledge from Africa. Bonesetting is practiced by many groups of West Africa (the Akan, Mano, and Yoruba, to name a few). In Djenné the mosquito was identified to be the cause of malaria, and the removal of cataracts was a common surgical procedure (as in many other parts of Africa).
On his return to the United States in 1890, Park worked on the bacteriology of diphtheria with Dr. Prudden. In 1893, Dr. Hermann Biggs, Professor of Bacteriology at New York University and Chief Inspector of the New York City Board of Health, offered Park a director's position in the municipal laboratories to continue his work on diphtheria. In 1894, Dr. Biggs telegraphed Park with the news of the discovery of the diphtheria antitoxin by Drs. Emile Roux and Emil von Behring and instructed him to begin inoculating horses to produce antitoxin in New York City.
After government felling of large stands of native forest, yellow fever was isolated from a patient from Cumaca in the northern range in 1954. The infection soon spread to other humans and into the Aedes aegypti mosquito population, greatly increasing transmission. Warnings were made that an epidemic was imminent and Dr. Wilbur Downs and Dr. A. E. (Ted) Hill, a specialist in tropical medicine, began a program of inoculating health workers and stockpiling vaccine. Trinidad health authorities followed up with large-scale vaccination and intensive anti- aegypti measures including public education, regular inspection for breeding sites, and spraying of domestic residences with DDT.
Secret Agent OSS 117, Colonel Hubert Bonisseur de La Bath (Kerwin Mathews) is sent to Thailand following the murder of OSS agent Christopher Lemmon. Lemmon had been investigating the break out of plague epidemics in India following health workers inoculating the locals to protect them from cholera. Lemmon had discovered that the medicine made in Hogby Laboratories in Bangkok had been switched with plague germs infecting the population with fatal results. After surviving several assassination attempts, OSS 117 infiltrates the secret lair of Dr. Sinn (Robert Hossein), an Indian hypnotist and psychologist with the help of his sister Lila (Pier Angeli).
As an intern in Buenos Aires in 1892, Alejandro Posadas described an Argentine soldier that had a dermatological problem since 1889. Posadas had seen the patient while a medical student in 1891 and skin biopsies revealed organisms resembling the protozoan Coccidia. The patient died in 1898 but during the interim Posadas successfully transmitted the infection to a dog, a cat, and a monkey, by inoculating them with material from his patient. In 1899 a 40 year old manual laborer from the San Joaquin Valley, a native of the Azores, entered a San Francisco hospital with fungating lesions similar to those of Posadas' patient.
They are useful for screening large numbers of people and testing people who cannot access healthcare facilities, but their sensitivity is relatively low, and it is recommended that a second method is used to confirm a positive result. T. cruzi can be isolated from samples through blood culture or xenodiagnosis, or by inoculating animals with the person's blood. In the blood culture method, the person's red blood cells are separated from the plasma and added to a specialized growth medium to encourage multiplication of the parasite. It can take up to six months to obtain the result.
In 1878, Albert Neisser isolated and visualized N. gonorrhoeae diplococci in samples of pus from 35 men and women with the classic symptoms of genitourinary infection with gonorrhea – two of whom also had infections of the eyes. In 1882, Leistikow and Loeffler were able to grow the organism in culture. Then in 1883, Max Bockhart proved conclusively that the bacterium isolated by Albert Neisser was the causative agent of the disease known as gonorrhea by inoculating the penis of a healthy man with the bacteria. The man developed the classic symptoms of gonorrhea days after, satisfying the last of Koch's postulates.
Louis Pasteur inoculating sheep against anthrax Anthrax posed a major economic challenge in France and elsewhere during the 19th century. Horses, cattle, and sheep were particularly vulnerable, and national funds were set aside to investigate the production of a vaccine. Noted French scientist Louis Pasteur was charged with the production of a vaccine, following his successful work in developing methods which helped to protect the important wine and silk industries. In May 1881, Pasteur – in collaboration with his assistants Jean-Joseph Henri Toussaint, Émile Roux and others – performed a public experiment at Pouilly-le-Fort to demonstrate his concept of vaccination.
The influential Massachusetts preacher Cotton Mather was the first known person to attempt smallpox inoculation on a large scale, inoculating himself and over 200 members of his congregation with the help of a local doctor. While his view became standard, he also caused the first reaction against the practice. Rowland Hill (1744–1833) was a popular English preacher acquainted with Edward Jenner, the pioneer of smallpox vaccination, and he encouraged the vaccination of the congregations he visited or preached to. He published a tract on the subject in 1806, at a time when many medical men refused to sanction it.
The Cǎoqū (草麴, lit. "herb ferment") entry in the Nanfang caomu zhuang is the earliest description of wine production using a natural ferment made with rice flour and herbs. In Southern Chinese tradition, this special rice wine was prepared in advance for marriage ceremonies while the daughter was still young. The traditional fermentation starter for Chinese wine is called jiǔqū (酒麴 "wine ferment", compounding jiǔ "wine; liquor" and qū "leaven; yeast") or jiǔyào (酒藥 "wine medicament", with yào "drug; medicine"), which is produced by inoculating a cereal dough with a previously-grown microbial culture (Needham and Huang 2000: 183).
Dimsdale developed a particular interest in the prevention of smallpox by inoculation (variolation), a deliberate infection of the patient via the skin with a mild form of the disease to give protection against more virulent strains. He published The present method of inoculating for the small-pox in 1767 which went into five editions by 1769. That year he was elected a Fellow of the Royal Society. In 1762, perhaps due to his reputation within London society, he was invited to Russia to variolate the Empress Catherine the Great of Russia and her son, Grand Duke Paul.
For reasons unknown, Sputnik is unable to replicate and produce new virions in these bald viruses. Virophage growth is deleterious to APMV and results in the production of abortive forms and abnormal capsid assembly of APMV. In one of the experiments done by inoculating Acanthamoeba polyphaga with water containing an original strain of APMV, it was discovered that several capsid layers accumulate asymmetrically on one side of the viral particle causing the virus to become ineffective. Sputnik decreased the yield of infective viral particle by 70% and also reduced the amoeba lysis by threefold at 24h.
One article by Douglass darkly joked about using smallpox in inoculations to kill off surrounding native communities. Inoculation made Boylston notorious, and a colonial government that rejected the notion of inoculation was deeply skeptical of his and Mather's experiment. The Boston City Council summoned Boylston in early August to explain, and the Council condemned inoculation and ordered him to desist immediately. Despite the opposition, Boylston garnered support of local learned men like Cotton's father Increase Mather and four other "inoculation ministers" (Benjamin Coleman, Thomas Prince, John Webb, and William Cooper) and defiantly resumed inoculating patients two day later.
Surgeon-Major Edward Nicholson wrote in the November 1870 Madras Medical Journal that he had witnessed a Burmese snake-catcher inoculating himself with cobra venom. However, the snake-catcher was unsure whether this was actually effective and therefore continued to treat his snakes with care. Nicholson, along with other Britons, began to consider that venom might provide its own cure. Although Scottish surgeon, Patrick Russell, had noted in the late 18th century that snakes were not affected by their own venom, it was not until the late 19th century that Joseph Frayer, Lawrence Waddell, and others began to consider venom-based remedies again.
A. bisporus being cultivated The earliest scientific description of the commercial cultivation of A. bisporus was made by French botanist Joseph Pitton de Tournefort in 1707. French agriculturist Olivier de Serres noted that transplanting mushroom mycelia would lead to the propagation of more mushrooms. Originally, cultivation was unreliable as mushroom growers would watch for good flushes of mushrooms in fields before digging up the mycelium and replanting them in beds of composted manure or inoculating 'bricks' of compressed litter, loam, and manure. Spawn collected this way contained pathogens and crops commonly would be infected or not grow at all.
The disease’s widespread dominance is conducive to high levels of variance between isolates, as many are separated by significant geographical distance and develop unique mutations. Variance between strains is due to sequence differentiation in the coat protein gene, occasionally leading to a different immune response. Therefore, detection of different strains is performed via genome sequencing or serology, which is made possible by inoculating rabbits with the virus. There are four strains currently known: EA, found only in East Africa; RC, found in Australia, Africa, North America, and Asia; O, from Africa, Asia, and South America; and C, from Australia, Africa, Asia, North America, and South America.
Cervical cancer nearly always involves human papillomavirus (HPV) infection. HPV is a virus with numerous strains, several of which predispose to precancerous changes in the cervical epithelium, particularly in the transformation zone, which is the most common area for cervical cancer to start. HPV vaccines, such as Gardasil and Cervarix, reduce the incidence of cervical cancer, by inoculating against the viral strains involved in cancer development. Potentially precancerous changes in the cervix can be detected by cervical screening, using methods including a Pap smear (also called a cervical smear), in which epithelial cells are scraped from the surface of the cervix and examined under a microscope.
At first, representatives of the Chinese community had agreed that inoculating the population with such serum could be a reasonable and safe solution, but soon after agreed with the rest of the Chinese population in that it was not ethical to try the vaccine in humans first. The representatives from the Chinese Six companies demanded the vaccination program to be eliminated as an option, and with much pressure and insistence from the Chinese community the vaccination program was halted. However, by then hundreds of Chinese, Japanese, other residents had already received the vaccine and were showing signs of horrific side effects from the injection.
A. Fleming (1969), "The Myth of the Mother Goddess" , World Archaeology 1(2), 247–261. Cynthia Eller also discusses the place of Gimbutas in inoculating the idea into feminism in her 2000 book The Myth of Matriarchal Prehistory. The 2009 book Knossos and the Prophets of Modernism by Cathy Gere examines the political influence on archaeology more generally. Through the example of Knossos on the island of Crete, which had been represented as the paradigm of a pacifist, matriarchal and sexually free society, Gere claims that archaeology can easily slip into reflecting what people want to see, rather than teaching people about an unfamiliar past.
Weigl worked on Rickettsia prowazekii and by inoculating lice and maintaining them on volunteers, he had developed a vaccine against tick typhus. In 1939, after the Nazi invasion of Poland, Maramorosch and his Warsaw-born wife Irene (née Ludwinowska) fled to Romania, where both were interned in Polish refugee camps for the following four years. After the liberation of Romania by the Soviet army Maramorosch continued his graduate studies at the Bucharest Polytechnic, choosing plant pathology as his major. In 1947 Karl and Irene emigrated to the United States, where Irene became a librarian in the New York Public Library, where she worked for the following 30 years.
At the end of 1926, funds were granted for further research into the cause of Dutch elm disease. Buisman was charged with this two-year project, and part of the villa garden was duly planted with elm seedlings. To infect so many plants, Buisman experimented with the use of a syringe, a method which would be used in successive decades. In 1927, she succeeded in producing both vascular discolouration and leaf wilt, simply by inoculating her trial plants earlier in summer than Bea Schwarz had done in 1921, confirming the results achieved by Wollenweber and Stapp in Berlin, providing the definitive proof that Graphium ulmi caused Dutch elm disease (DED).
Nevertheless, GBS-like colonies that develop in chromogenic media should be confirmed as GBS using additional reliable tests to avoid mis-identification. Inoculating directly the vaginal and rectal swabs or the vaginorectal swab in a plate of an appropriate culture medium (blood agar, granada medium or chromogenic media) is also possible. However, this method (bypassing the selective enrichment broth step) can lead to some false-negative results, and this approach should be taken only in addition to, and not instead of, inoculation into selective broth. Today, in the UK, the detection of GBS colonization using the enrichment broth technique is not offered from most laboratories serving the NHS.
Doctors, ministers, laymen, and Boston city officials argued that the practice of inoculating healthy individuals would spread the disease and that it was immoral to interfere with the working of divine providence. Mather was also publicly ridiculed for relying on the testimony of a slave. It was commonly anticipated that enslaved Africans would attempt an overthrow of white society; therefore Onesimus’ medicinal wisdom was met with severe mistrust and assumed to be a ploy to poison white citizens. The Acts and Resolves passed in Boston, which included race-based punishments and codes to prevent slave or servant uprisings (because Bostonians feared conspiracy and conflict), showed a society skeptical of African medicine.
There is continued disagreement over what "innate" and "subluxation" mean to chiropractic. Some chiropractors believe in Innate Intelligence, a faith-based, unscientific belief which has been a source of derision for chiropractors. Chiropractors historically were strongly opposed to vaccination based on their belief that all diseases were traceable to causes in the spine, and therefore could not be affected by vaccines; D.D. Palmer wrote, "It is the very height of absurdity to strive to 'protect' any person from smallpox or any other malady by inoculating them with a filthy animal poison." There is significant disagreement within the chiropractic community concerning vaccination, one of the most cost-effective public health interventions available.
However, the primary object of the Institute in 1895 was to cultivate the pleuro-pneumonia virus for inoculating cattle. In 1897 administration of the Institute passed from the Colonial Secretary's Office to the Queensland Department of Agriculture, founded in 1887. In 1899 new premises for the Institute were constructed in the then Brisbane suburb of Normanby (now within Spring Hill). The two-storey red brick building, located next to the Brisbane Grammar School residence on College Road, included rooms for Mr Pound and clerical work; a general laboratory; plus chemistry, incubator, sterilizing and photographic rooms on the ground floor, and a museum, library and lecture room on the first floor.
He was particularly concerned with epidemic disease, repeatedly petitioning town authorities in Wallingford and Middletown for permission to establish a "pock house" for inoculating against small pox. Hall's anti- federalist political inclinations may have helped him to develop a relationship with Boston physician Benjamin Waterhouse, a correspondent of British physician Edward Jenner, discoverer of vaccination. Jenner sent samples of cowpox matter to Waterhouse, some of which may have been passed on to Hall, who appears to have been the first physician in Connecticut to practice vaccination. In 1796, Hall played a heroic role during a yellow fever epidemic at Knowles Landing, south of Middletown on the Connecticut River.
The first written report of rinderpest inoculation was published in a letter signed "T.S." in the November 1754 issue of The Gentleman's Magazine, a widely read journal which also supported the progress of smallpox inoculation. This letter reported that a Mr Dobsen had inoculated his cattle and had thus preserved 9 out of 10 of them, although this was retracted in the next issue, as it was apparently a Sir William St. Quintin who had done the inoculating (this was done by placing bits of material previously dipped in morbid discharge into an incision made in the dewlap of the animal). These letters encouraged further application of inoculation in the fight against diseases.
This and other work paved the way for Mortimer Cromwell, a secretary of the Royal Society, to raise plagues as a national health issue enabling a general policy of quarantine, isolation, fumigation, and slaughter. Erasmus Darwin was also impacted by the tragedy of the plagues and it resulted in the publication of his Zoonomia where he discusses infectious disease of both humans and animals. In 1802 French physiologist François Magendie (1783 - 1855) became the first person to prove interspecies transmission of disease by inoculating a dog from rabies using human spittle. He also experimented with the injection of putrid fish into animals and was an advocate for experimentation in a time before anesthetics were developed.
Species in the genus Echinodontium have a hydnaceous (with spinelike protuberances) hymenial surface, dimitic hyphal system, smooth basidiospores, and in three of four species, a symbiotic association with horntails — Sirex and Urocerus species. The insects eat the fungus and help disperse the species by inoculating the wood of the host tree with either hyphal fragments or spores when they lay their eggs. The genus Laurilia has species characterized by an even to tuberculate hymenial surface, dimitic hyphal system, and echinulate basidiospores. In his monograph on the Echinodontiaceae, Gross (1964) suggested that Echinodontium and Laurilia were congeneric (sharing the same taxonomic genus), sharing features such as clamp connections, thick-walled encrusted cystidia and a brown context.
While working at the Rockefeller Institute of Medical Research in 1911, he was accused of inoculating orphan children with syphilis in the course of a clinical study."BASELESS CHARGE AGAINST DR. NOGUCHI; Libel Action May Arise Out of a Charge by the Anti-Vivisectionists", New York Times He was acquitted of any wrongdoing at the time but, since the late 20th century, his conduct of the study has come to be considered an early instance of unethical human experimentation. At the time, society had not developed a consensus about how to conduct human experimentation and feelings varied about the medical research community. Antivivisectionists linked their concerns for animals with concerns about humans.
Uncertainties have existed of B. pertussis and whooping cough as a zoonotic disease since around 1910 but in the 1930s, knowledge was gained that the bacteria lost their virulent power when repeatedly spread on agar media. This explained the difficulties to reproduce results from different studies as the pre-inoculating handlings of the bacteria were not standardized among scientists. Today it is established that at least some primate species are highly susceptible to B. pertussis and develop clinical whooping cough in high incidence when exposed to low inoculation doses. The bacteria may be present in wild animal populations, but this is not confirmed by laboratory diagnosis, although whooping cough is known among wild gorillas.
Commercially available home yogurt maker Yogurt is made by heating milk to a temperature that denaturates its proteins (scalding), essential for making yogurt, cooling it to a temperature that will not kill the live microorganisms that turn the milk into yogurt, inoculating certain bacteria (starter culture), usually Streptococcus thermophilus and Lactobacillus bulgaricus, into the milk, and finally keeping it warm for several hours. The milk may be held at for a few minutes, or boiled (giving a somewhat different result). It must be cooled to or somewhat less, typically . Starter culture must then be mixed in well, and the mixture must be kept undisturbed and warm for some time, anywhere between 5 and 12 hours.
A former Captain in the Japanese Maritime Self-Defense Force, Kaito Takahaya became a pirate after the Red Flu outbreak. He and his group took members of the USS Nathan James (DDG-151) prisoner when his people begin to die after being administered the cure by US soldiers. He is captured by the Nathan James during their rescue of the POWs, and CAPT Slattery is able to turn him into an ally against Chinese President Peng, once it is revealed that Peng was behind inoculating the Japanese people against the cure in an attempt at genocide. After his son was inoculated, he aids the James in bringing him to justice at the end of a katana.
His account of the incident was not publicly questioned during his lifetime nor for more than a century after his death. However, in recent years, his version of the event has been called into question by many historians. Holwell's monument in Calcutta Holwell has also become an important source for modern historians of medicine, as a result of his description of the practice of smallpox variolation in eighteenth-century Bengal, An Account of the Manner of Inoculating for the Small Pox in the East Indies with Some Observations on the Practice and Mode of Treating that Disease in those Parts (London, 1767). Born in Dublin, he grew up in London, and studied medicine at Guy's Hospital.
Malick, however, later uses the same trick to avoid being sent to Maveth, ensuring Nathaniel is sent instead, who's horrified at his brother's betrayal. In the present, realising that the Inhuman from Maveth is possessing Ward's body, Fitz and Simmons determine that the Transia board members were eaten alive by a microscopic insectoid swarm, deducing that the Inhuman is made up of an amalgam of these alien organisms. Coulson is left disillusioned that he may have unleashed a far worse threat by killing Ward. Hydra seize control of an agricultural company related to Transia, who are studying means of inoculating animals and plants against invasive organisms, leading Simmons to theorise that their studies could be used against the Inhuman.
This table displays the steps involved in using a GasPak to create a completely anaerobic environment for incubating an inoculated agar plate. After inoculating the agar plate(s) with bacteria under aseptic conditions, the agar plates are placed in an anaerobic jar that contains components, like the catalyst chamber, that will help facilitate the reaction to eliminate free oxygen and provide completely anaerobic conditions. The methylene blue strip is an indicator that is placed in the jar that will turn colorless under entirely anaerobic conditions, which provides proof that the jar was able to successfully reach an anaerobic state. The components of the GasPak envelope along with the addition of water, allow for the start of the reaction that takes place in the catalyst chamber.
Jan (or John) Ingenhousz or Ingen-Housz FRS (8 December 1730 – 7 September 1799) was a Dutch physiologist, biologist and chemist. He is best known for discovering photosynthesis by showing that light is essential to the process by which green plants absorb carbon dioxide and release oxygen.Beale and Beale, Echoes of Ingen Housz, 2011Geerd Magiels, Dr. Jan Ingenhousz, or why don't we know who discovered photosynthesis, 1st Conference of the European Philosophy of Science Association 2007 He also discovered that plants, like animals, have cellular respiration. In his lifetime he was known for successfully inoculating the members of the Habsburg family in Vienna against smallpox in 1768 and subsequently being the private counsellor and personal physician to the Austrian Empress Maria Theresa.
The primary way to boost levels of immunity in a population is through vaccination. Vaccination is originally based on the observation that milkmaids exposed to cowpox were immune to smallpox, so the practice of inoculating people with the cowpox virus began as a way to prevent smallpox. Well- developed vaccines provide protection in a far safer way than natural infections, as vaccines generally do not cause the diseases they protect against and severe adverse effects are significantly less common than complications from natural infections. The immune system does not distinguish between natural infections and vaccines, forming an active response to both, so immunity induced via vaccination is similar to what would have occurred from contracting and recovering from the disease.
In the Bahamas, where they are locally known as money moths or money bats, the legend is that if they land on you, you will come into money, and similarly, in South Texas, if a black witch lands above your door and stays there for a while, you will supposedly win the lottery. The Black Witch Moth: Its Natural & Cultural History, by Mike Quinn In Paraguay, people are afraid of the black witch, as there is a mistaken belief about the moth urinating over their human "victims" and thereby inoculating their eggs, which then develop into maggots developing under the skin. Some also believe, if it touches your eyes, you can go blind. The maggots referred to are the myiasis-causing larvae of the human botfly (Dermatobia hominis).
He studied mortality statistics for London for the fourteen years prior to 1723 and concluded that one fourteenth of the population had died from smallpox, up to 40 percent during epidemics. He advertised in the Proceedings of the Royal Society for readers to report their personal and professional experiences and received over sixty replies, most from other physicians or surgeons, but most significantly from Thomas Nettleton who reported his own calculations from his experience in several communities in Yorkshire. Jurin's analysis concluded that the probability of death from variolation was roughly 1 in 50, while the probability of death from naturally contracted smallpox was 1 in 7 or 8. He published his results in a series of annual pamphlets, An Account of the Success of Inoculating the Small-Pox (1723–1727).
This can be desirable if cider makers prefer their cider to have some more sugar than alcohol in their cider. However, limiting YAN should be done in moderation because too little nitrogen can lead to an increase in H2S production; H2S is responsible for a rotten egg-like smell. Primary cider fermentation can be initiated by inoculating the cider must with selected yeast strains or by permitting indigenous yeast strains present on the fruit and in the cider production equipment to spontaneously commence fermentation without inoculation. Inoculation with different strains of Saccharomyces cerevisiae and other yeast strains with strong fermentative metabolism traits, including Saccharomyces bayanus and Torulaspora delbrueckii strains, has been shown to produce few differences in cider phenolic compounds, save for concentrations of phloretin (see Phloretin) in samples that underwent malolactic fermentation.
The earliest recorded artificial induction of immunity in humans was by variolation or inoculation, which is the controlled infection of a subject with a less lethal natural form of smallpox (known as Variola Minor) to make him or her immune to re-infection with the more lethal natural form, Variola Major. This was practiced in ancient times in China and India, and imported into Europe, via Turkey, around 1720 by Lady Montagu and perhaps others. From England, the technique spread rapidly to the Colonies, and was also spread by African slaves arriving into Boston. Variolation had the disadvantage that the inoculating agent used was still an active form of smallpox and, although less potent, could still kill the inoculee or spread in its full form to others nearby.
The editorial stance was that the Boston populace feared that inoculation spread, rather than prevented, the disease; however, some historians, notably H. W. Brands, have argued that this position was a result of the contrarian positions of editor-in-chief James Franklin (a brother of Benjamin Franklin). Public discourse ranged in tone from organized arguments by John Williams from Boston, who posted that "several arguments proving that inoculating the smallpox is not contained in the law of Physick, either natural or divine, and therefore unlawful", to those put forth in a pamphlet by Dr. William Douglass of Boston, entitled The Abuses and Scandals of Some Late Pamphlets in Favour of Inoculation of the Small Pox (1721), on the qualifications of inoculation's proponents. (Douglass was exceptional at the time for holding a medical degree from Europe.) At the extreme, in November 1721, someone hurled a lighted grenade into Mather's home.
A thermal insulation panel ready for installation, 'Mushroom materials' are a novel class of renewable biomaterial grown from fungal mycelium and low-value non-food agricultural materials using a patented process developed by Ecovative Design. After being left to grow in a former in a dark place for about five days during which time the fungal mycelial network binds the mixture, the resulting light robust organic compostable material can be used within many products, including building materials, thermal insulation panels and protective packaging. The process uses an agricultural waste product such as cotton hulls, cleaning the material, heating it up, inoculating it to create growth of the fungal mycelium, growing the material for period of about five days, and finally heating it to make the fungus inert. During growth, the material's shape can be molded into various products including protective packaging, building products, apparel, car bumpers, or surfboards.
Kinepox is an alternative term for the smallpox vaccine used in early 19th-century America. Popularized by Jenner in the late 1790s, kinepox was a far safer method for inoculating people against smallpox than the previous method, variolation, which had a 3% fatality rate. In a famous letter to Meriwether Lewis in 1803, Thomas Jefferson instructed the Lewis and Clark expedition to "carry with you some matter of the kine-pox; inform those of them with whom you may be, of its efficacy as a preservative from the smallpox; & encourage them in the use of it..." Jefferson had developed an interest in protecting Native Americans from smallpox, having been aware of epidemics along the Missouri River during the previous century. A year before his special instructions to Lewis, Jefferson had persuaded a visiting delegation of North American Indian chieftains to be vaccinated with kinepox during the winter of 1801–1802.
Prior to joining the Italian Parliament, Capua served as director of the Department of Comparative Biomedical Sciences for the Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Legnaro in Padua. The department is home to the National FAO/OIE Reference Laboratory for Avian Influenza and Newcastle Disease, and is the OIE Collaborating Centre for Diseases at the Animal/Human Interface. In 1999-2000, responding to a persistent but relatively non- virulent strain of H7N1 avian flu hindering Italy’s commercial poultry industry, Capua and collaborators developed an innovative approach, which was dubbed the "DIVA" strategy (Differentiating Infected from Vaccinated Animals) as a tool to support eradication practices. The strategy involved inoculating poultry with an inactivated vaccine derived from an antigenically related H7N3 virus—coupled with a diagnostic test directed to identifying antibodies to the neuramminidase antigen, that revealed whether avian-flu antibodies present in a subject animal were caused by the H7N3 vaccine or by the field H7N1 virus.

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