Many of the salamander species tested fluoresced more on their undersides than backs, perhaps an adaptation to keep predators above from noticing them.
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That distantly related species of amphibians all fluoresced suggests that the trait likely developed early in amphibian evolution and may be quite widespread among the group.
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Not only that, but the fracture zones were partially coated with a carbonate mineral that fluoresced a pastel bluish-white and phosphoresced a pale greenish-white.
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Kiss-and-run fusion has been thought to be stabilized by an actin coating of vesicles. Testing for the vesicle uptake of FM1-43 to note when vesicles fused with the membrane allowed researchers to notice that actin coating is a necessary step for the kiss-and-run mechanism. Vesicles labelled with the Beta-actin-green fluorescent protein (GFP) fluoresced seconds after fusing with the presynaptic membrane (as shown by FM1-43 uptake), but non-fused vesicles never fluoresced, suggesting that an actin coating is required for kiss-and-run. This actin coating came from the polymerization of actin monomers.
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In 2019 it was observed, by chance, that a flying squirrel fluoresced pink. Subsequent research by biologists at Northland College in Northern Wisconsin found that this is true for all three species of North American flying squirrels. At this time it is unknown what purpose this serves. Non- flying squirrels do not fluoresce under UV light.
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Aerolux gas discharge light bulbs contained low pressure gas, either neon or argon, or a mixture of the two. Also within the bulb were metal sculptures coated with phosphors. These phosphors fluoresced when excited by glow discharge. Because glow discharge occurs readily at 110-120 volts AC, one could use these bulbs in standard household lamps in the United States.
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Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and the epifluorescence method therefore gives a high signal-to-noise ratio. The dichroic beamsplitter acts as a wavelength specific filter, transmitting fluoresced light through to the eyepiece or detector, but reflecting any remaining excitation light back towards the source.
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Schizosaccharomyces pombe fluoresced with dihydroethidium Microfluorimetry is an adaption of fluorimetry for studying the biochemical and biophysical properties of cells by using microscopy to image cell components tagged with fluorescent molecules. It is a type of microphotometry that gives a quantitative measure of the qualitative nature of fluorescent measurement and therefore, allows for definitive results that would have been previously indiscernible to the naked eye.
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This combination yielded large amounts of organic material that self-organised to form globules or vesicles when immersed in water. The investigating scientist considered these globules to resemble cell membranes that enclose and concentrate the chemistry of life, separating their interior from the outside world. The globules were between , or about the size of red blood cells. Remarkably, the globules fluoresced, or glowed, when exposed to UV light.
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Hale both cataloged the presence of chemicals from numerous North American species and described new chemicals. One example, is Hale's study on fluorescence in which he linked fluoresced colors to specific chemicals using paper chromatography. In addition to chemistry, Hale also incorporated scanning electron microscopy characteristics such as cortical structure into his species concepts. Another technical advance Hale utilized was punch card computers to keep track of morphological and ecological data.
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By building laser systems in the laboratory, Jackson helped to establish the excited states of molecules that are present in planetary atmospheres. The experiments consisted of one laser for the photodissociation of the parent molecule, and another laser to excite the free radical into an excited state. When the excited molecule fluoresced back to the ground state, the fluorescence was captured in a photomultiplier tube. He has investigated the photochemistry of carbon monoxide, nitrogen and carbon dioxide.
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Raw CCD frames are processed into data products on board the rover to reduce the data volume. These data products are transmitted to Earth for further processing analyses. In operation, a collimated X-ray source produces and directs a beam through a transmission sample cell containing powdered material. An X-ray sensitive CCD (charge-coupled device) imager is positioned on the opposite side of the sample from the source and directly detects X-rays diffracted or fluoresced by the sample.
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In the last years of the 19th century, scientists frequently experimented with the cathode-ray tube, which by then had become a standard piece of laboratory equipment. A common practice was to aim the cathode rays at various substances and to see what happened. Wilhelm Röntgen had a screen coated with barium platinocyanide that would fluoresce when exposed to cathode rays. On 8 November 1895, he noticed that even though his cathode-ray tube was not pointed at his screen, which was covered in black cardboard, the screen still fluoresced.
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Fluorapatite as a naturally occurring impurity in apatite generates hydrogen fluoride as a byproduct during the production of phosphoric acid, as apatite is digested by sulfuric acid. The hydrogen fluoride byproduct is now one of the industrial sources of hydrofluoric acid, which in turn is used as a starting reagent for synthesis of a range of important industrial and pharmaceutical fluorine compounds. Synthetic fluorapatite doped with manganese-II and antimony-V formed the basis for the second generation of fluorescent tube phosphors referred to as halophosphors. When irradiated with 253.7 nm mercury resonance radiation they fluoresced with broad emission which appeared within the range of acceptable whites.
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This allowed for a simple bioconjugation to tag the organisms with the quantum dots, by taking advantage of the high affinity interaction between streptavidin and biotin. D. magna individuals were successfully tracked with each having a unique quantum dot that fluoresced and emitted light of a specific wavelength that could be detected using cameras. The tags were useful for up to 24 hours, after which the organisms shed their carapace containing the quantum dot. Better cameras are being developed that will improve the depth at which the quantum dot- tagged organisms can be observed and allow for studies to be conducted in natural environments.
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The fluoresced microbe can then be subjected to analysis and cell sorting. The detection phase involves putting the detected sample to “functional gene analysis to indicate specific separation using 16S rRNA, pmoA (encoding a subunit of particulate methane monooxygenase), and fae (encoding formaldehyde activating enzyme) genes.”Kalyuzhnaya MG, Zabinsky R, Bowerman S, Baker DR, Lidstrom ME, Chistoserdova L. “Fluorescence In Situ Hybridization-Flow Cytometry-Cell Sorting-Based Method for Separation and Enrichment of Type I and Type II Methanotroph Populations” Applied and Environmental Microbiology. 2006. Vol 72, No. 6. p. 4293-4301. The data indicate that FISH/FC/FACS is a method that can “provide significant enrichment of microbial populations of interest from complex natural communities.” Lastly, Kalyuzhaya et al.
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