112 Sentences With "dissociate from" | Random Sentence Generator

You can't and shouldn't dissociate from what's happening in Washington.

But a year after the operation, he began to dissociate from his previous self.

Here it's possible to mostly dissociate from their former lives and think only about the future.

The American-backed rebels have been told to dissociate from all who are not part of the truce.

As someone who never feels like enough, it's easy for me to dissociate from a moment—even a delicious one.

After allegations of Bill Cosby's sexual assaults surfaced, Spelman sought to dissociate from its long-standing relationship with the performer.

We see this in the constituency his plans appeal to and in his reluctance to dissociate from the racist demographic of his supporters.

But a handful of Democrats want to be able to dissociate from Pelosi, raising the chances of ushering new leadership in the next Congress.

The white women of the left, many of whom are just now finding their footing as activists, have been eager to dissociate from that group.

"The Bolsonaro government will dissociate from the Global Compact for Migration ... an inappropriate instrument to deal with the problem," Araújo wrote on Twitter late that same say.

If Big Oil becomes universally regarded as toxic, there would be immense public pressure for legislators to dissociate from it, much as they have with other disgraced industries.

It was intoxicating—not just in the literal sense of opiate euphoria—but also because heroin allowed me to dissociate from everything and everyone but my own inner world.

But most of all, they reveal a movement that, much like Trump himself, finds itself isolated — trying desperately to dissociate from the convenient alliances it made in the campaign, and in danger of irreparably tarnishing its credibility.

These giant consumer companies can be bad actors on their own, but for them to attempt to dissociate from bad actors who happen to be consuming their products, especially actual actors, in the case of Apple, seems like misplaced concern.

Usually, when people take the animal tranquilizer ketamine—also known simply as "k" or "special K"—it's to dissociate from their own bodies and vanish down the "K-hole," which can include dissociative feelings of euphoria and serious short-term memory loss (... or so we hear).

After riots in the Paris suburbs that year, Muslim youths felt a "need to dissociate from France, and leave it," he wrote in his book "Terreur dans l'Hexagone," which appeared soon after the Paris attacks in November and sold tens of thousands of copies to a public hungry for explanation.

Mr. Hariri said in a brief statement after a cabinet meeting Tuesday that the government had recommitted to dissociate "from any dispute and conflicts or wars, and not to interfere in the internal affairs of the Arab states, in order to preserve the relationship between Lebanon and its Arab brethren."

It may have had something to do with that Glee episode, or that season of American Horror Story, the reemergence of witchcraft in popular culture, or the collective desire of young urban Millennials to completely dissociate from contemporary society, which sucks, and redirect their attention to collecting crystals, eating magic dust, studying tarot, and pretending that we were all so goth for the entirety of our teen years.

The result, two weeks before Election Day, has been the central strategic divide across several midterm battlegrounds: a Democrat keeping the focus local, hoping to dissociate from divisive national party figures like Chuck Schumer and Nancy Pelosi; and a Republican eager to make the race a referendum on Mr. Trump and the leftist "mob" opposing him, betting that the risk of a volatile and meandering executive messenger is worth the reward of an energized base.

This not only altered the patient's behavioral pattern but also made them dissociate from reality during that particular time frame.

Now insulin molecules spontaneously dissociate from the autoantibodies, giving rise to a raised free insulin level inappropriate for the glucose concentration, causing hypoglycemia.

Nitromifene has been found to dissociate from the estrogen receptor 250-fold faster than estradiol. This may be involved in its antagonistic activity at the estrogen receptor.

The preinitiation complex, which contains mediator, transcription factors, a nucleosome and RNA polymerase II, is important to position the polymerase for the start of transcription. Before RNA synthesis can occur, the polymerase must dissociate from mediator. This appears to be accomplished by phosphorylation of part of the polymerase by a kinase. Importantly, mediator and transcription factors do not dissociate from the DNA at the time polymerase begins transcription.

" :Kumayl: "Yes, I love Abu Turab." :Hajjaj: "Curse be on you and on Abu Turab. Dissociate from Abu Turab and I will let you go." :Kumayl: "Show me a way better than Abu Turab's way.

Heme C differs from heme B in that the two vinyl side chains of heme B are replaced by covalent, thioether linkages to the apoprotein. The two thioether linkages are typically made by cysteine residues of the protein. These linkages do not allow the heme C to easily dissociate from the holoprotein, cytochrome c, compared with the more easily dissociated heme B that may dissociate from the holoprotein, the heme-protein complex, even under mild conditions. This allows a very wide range of cytochrome c structure and function, with myriad c type cytochromes acting primarily as electron carriers.

On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the cytochrome P450 system genes.

The process of spliceosome formation involves the U4 and U6 snRNPs associating and forming a di-snRNP in the cell nucleus. This di-snRNP then recruits another member (U5) to become a tri-snRNP. U6 must then dissociate from U4 to bond with U2 and become catalytically active. Once splicing has been done, U6 must dissociate from the spliceosome and bond back with U4 to restart the cycle. Prp24 has been shown to promote the binding of U4 and U6 snRNPs. Removing Prp24 results in the accumulation of free U4 and U6, and the subsequent addition of Prp24 regenerates U4/U6 and reduces the amount of free U4 and U6.

In 1922, he married Katherine Eleanor Mackenzie. They had a daughter. In June 1936, Adélard Godbout succeeded Louis-Alexandre Taschereau as premier of Quebec. Wanting to dissociate from old government, which was sullied by the scandals, Godbout sought out some non-politicians to serve in his cabinet.

Motifs for complexation of tri- and tetradentate tripodal ligands Tripodal ligands are tri- and tetradentate ligands with C3 symmetry. They are popular in research in the areas of coordination chemistry and homogeneous catalysis. Because the ligands are polydentate, they do not readily dissociate from the metal centre.

DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Phosphine ligands are usually "spectator" rather than "actor" ligands. They generally do not participate in reactions, except to dissociate from the metal center. In certain high temperature hydroformylation reactions, the scission of P-C bonds is observed however. The thermal stability of phosphines ligands is enhanced when they are incorporated into pincer complexes.

Overview of signal transduction pathways involved in apoptosis. Akt could promote growth factor-mediated cell survival both directly and indirectly. BAD is a pro-apoptotic protein of the Bcl-2 family. Akt could phosphorylate BAD on Ser136, which makes BAD dissociate from the Bcl-2/Bcl-X complex and lose the pro-apoptotic function.

When bound to GDP, G proteins are inactive. When a ligand binds a GPCR, an allosteric change in the G protein is triggered, causing GDP to leave and be replaced by GTP. GTP activates the alpha subunit of the G protein, causing it to dissociate from the G protein and act as a downstream effector.

The Young Czechs began to dissociate from the main party and radicalist actions subsided for a period of time. The National Liberal Party was banned from the local Diet and many newspapers were censored. Národní Listy, Javnost, and Omladina faced increasingly harsh government restrictions. The government increased severe measures against the minority and the Progressive movement was momentarily halted.

Specifically, the hydrolysis of PIP2 to IP3. This hydrolysis causes PIP2, which is bound to the membrane, to become IP3 and dissociate from the membrane into the cytoplasm. When M-current is restored, it moves back to the membrane. There is some evidence for different theories of how M-channel activity is directly affected by PIP2.

The symptoms of Pick's disease include difficulty in language and thinking, efforts to dissociate from family, behavioral changes, unwarranted anxiety, irrational fears, compulsive buying disorder (CBD or oniomania), impaired regulation of social conduct (e.g., breaches of etiquette, vulgar language, tactlessness, disinhibition, misperception), passivity, low motivation (aboulia), inertia, overactivity, pacing, and wandering.Semple, David. "Oxford Handbook of Psychiatry".

Like balls in a Newton's cradle, electrons in a metal quickly transfer energy from one terminal to another, despite their own negligible movement. A metal consists of a lattice of atoms, each with an outer shell of electrons that freely dissociate from their parent atoms and travel through the lattice. This is also known as a positive ionic lattice.Bonding (sl). ibchem.

Thus inhibited GSK3, allows β-catenin to dissociate from APC, accumulate, and travel to nucleus. In the nucleus β-catenin associates with Lef/Tcf transcription factor, which is already working on DNA as a repressor, inhibiting the transcription of the genes it binds. Binding of β-catenin to Lef/Tcf works as a transcription activator, activating the transcription of the Wnt-responsive genes.

DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.

The new bases eventually dissociate from the DNA bases but stay linked to each other, forming a new mRNA strand. This mRNA strand is synthesized in the 5’ to 3’ direction. Once the RNA reaches a terminator sequence, it dissociates from the DNA template strand and terminates the mRNA sequence as well. Transcription is regulated in the cell via transcription factors.

Some thiaminases are produced by bacteria. Bacterial thiaminases are cell surface enzymes that must dissociate from the membrane before being activated; the dissociation can occur in ruminants under acidotic conditions. Rumen bacteria also reduce sulfate to sulfite, therefore high dietary intakes of sulfate can have thiamine-antagonistic activities. Plant thiamine antagonists are heat-stable and occur as both the ortho- and para-hydroxyphenols.

E. S. Pankhurst 1931, p. 517. With their mother's blessing, Christabel ordered Sylvia's group to dissociate from the WSPU. Pankhurst tried to persuade the ELFS to remove the word "suffragettes" from its name, since it was inextricably linked to the WSPU. When Sylvia refused, her mother switched to fierce anger in a letter: > You are unreasonable, always have been & I fear always will be.

Four short ORF's exist in the leader of the GCN4 mRNA. 40S Ribosomal Subunits scanning the mRNA from 5' have TC bound and translate the first upstream open reading frame (uORF). Under non-starving condition there is enough ternary complex that the subunits rebind it before they reach uORF 4. Translation is again initiated, uORF2,3 or 4 translated and the 40S Subunits subsequently dissociate from GCN4 mRNA.

There are two classes of release factors. Class 1 release factors recognize stop codons; they bind to the A site of the ribosome in a way mimicking that of tRNA, releasing the new polypeptide as it disassembles the ribosome. Class 2 release factors are GTPases that enhance the activity of class 1 release factors. It helps the class 1 RF dissociate from the ribosome.

Every Shia Muslim believes it to be their duty to dissociate themselves from the enemies of God and his Messengers. Muslims differ on whom to consider to be the enemies of God, Muhammad and the Ahl al-Bayt. For brevity, only Ahl al-Bayt will be mentioned in this article.Tawalla and Tabarra The doctrine of Tabarra itself does not dictate whom to dissociate from or whom to associate with.

The few males that emerge in the first brood mate with workers that eventually leave the original nest to become queens in new nests. The last eggs are laid in late August–September and these males and future overwintering females emerge in late September–October. After a few weeks, the wasps dissociate from the nest to seek shelter for the winter. Some mating may occur in these shelters.

Only the Ol-zhaan know that the Kindar are descendants of a centuries-old colony escaping a world destroyed by war and violence. Society was carefully constructed to nurture positive emotions and repress negative "unjoyful" ones. Even little children practice mind-blocking and eat soothing Wissenberries to dissociate from forbidden emotions. Raamo learns that Genaa's father is dead, captured by the Pash-shan, feared creatures that live trapped below the ground.

If the brain registers an overwhelming trauma, then it can essentially block that memory in a process called dissociation or detachment from reality. "The brain will attempt to protect itself". The same way the body can wall-off an abscess or foreign substance to protect the rest of the body, the brain can dissociate from an experience. In the midst of trauma, the brain may wander off and work to avoid the memory.

Cell receptor disruption can also affect the ability for cells to adhere to one another with adherens junctions. Without new capillary formation, the existing capillaries break down and cells start to dissociate from each other. This in turn leads to the breakdown of gap junctions. Gap junctions in endothelial cells in the BBB help prevent large or harmful molecules from entering the brain by regulating the flow of nutrients to the brain.

By blocking AT1 receptors, ARBs lead to lower blood pressure. An insurmountable inhibition of the AT1 receptor is achieved when the maximum response of Ang II cannot be restored in the presence of the ARB, no matter how high the concentration of Ang II is. The angiotensin receptor blockers can inhibit the receptor in a competitive surmountable, competitive insurmountable or noncompetitive fashion, depending upon the rate at which they dissociate from the receptor.

A single recorded in January 1977, Madame Thibault becomes a number one in Quebec.Photo-Journal, April 9, 1977 A disco version, mostly instrumental except for the phrase "Ma'm Thibault", is created and becomes a club hit. The single is even issued in France and Australia, where it becomes very popular. The song credited to "Tranquille", to dissociate from the TV character, is also issued on a K-tel double-LP compilation called Disco Fever (1978).

At the age of 19, he went to Canada to harvest tobacco. Upon his return, with his brother Wim he brought new life to the inactive department of the People's Union (Volksunie) in Schaarbeek. In 1969, an activist of the so-called Democratic Front of Francophones (FDF) with a not very good health suffers from a heart attack during an action of the VMO. After this incident, the VU seems to dissociate from its activists.

This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.

In addition to TORC1, protein kinase A (PKA) inhibits autophagy through the phosphorylation of Atg1 and Atg13. PKA phosphorylates Atg1 at two distinct serine residues; these modifications were shown to be necessary for Atg1 to properly dissociate from the PAS. The downstream substrate of Atg1 kinase hasn't been described yet, and it is still a matter of debate as to whether Atg1 primarily acts on autophagy through its kinase activity or through a structural role during autophagic complex formation.

At high heart rates, phospholamban is phosphorylated and deactivated thus taking most from the cytoplasm back into the sarcoplasmic reticulum. Once again, calcium buffers moderate this fall in concentration, permitting a relatively small decrease in free concentration in response to a large change in total calcium. The falling concentration allows the troponin complex to dissociate from the actin filament thereby ending contraction. The heart relaxes, allowing the ventricles to fill with blood and begin the cardiac cycle again.

When heated beyond a certain energy level, electrons dissociate from their atoms entirely, producing a gas of nuclei and electrons known as a plasma. Plasma is a gas, and its energy causes it to adiabatically expand according to the ideal gas law. As it does, its temperature drops, eventually reaching a point where the electrons can reconnect to nuclei. The cooling process causes the bulk of the plasma to reach this temperature at roughly the same time.

Tauopathy belongs to a class of neurodegenerative diseases involving the aggregation of tau protein into neurofibrillary or gliofibrillary tangles (NFTs) in the human brain. Tangles are formed by hyperphosphorylation of the microtubule protein known as tau, causing the protein to dissociate from microtubules and form insoluble aggregates. (These aggregations are also called paired helical filaments.) The mechanism of tangle formation is not well understood, and whether tangles are a primary cause of Alzheimer's disease or play a peripheral role is unknown.

As two molecules of acetylcholine both bind to the binding sites on α subunits, the conformation of the receptor is altered and the gate is opened, allowing for the entry of many ions and small molecules. However, this open and occupied state only lasts for a minor duration and then the gate is closed, becoming the closed and occupied state. The two molecules of acetylcholine will soon dissociate from the receptor, returning it to the native closed and unoccupied state.

Garrett admitted she had a very unpleasant childhood and because of the anger of her aunt would "separate into a world of her own" where she could dissociate from her surroundings. She claimed to have developed psychic ability in her youth. She later married and claimed to hear voices and show symptoms of a dissociative identity disorder. Both Garrett and her husband believed she was on the "brink of madness", however, Garrett came to accept her condition and took up trance mediumship.

When a cell enters mitosis, Sororin is phosphorylated causing it to dissociate from cohesin meaning WAPL can remove cohesin from the DNA. A complex of SGOL1 and PP2A dephosphorylates cohesin at the centromere protecting it from WAPL-mediated release. Sister chromatid cohesion is therefore maintained at the centromeres where it is required for mitosis but lost on the arms. This removal of cohesin is known as the Prophase Pathway and results in the X-shape sister chromatids observed in chromosome spreads.

This structural change causes an increased affinity for the regulatory protein called transducin (a type of G protein). Upon binding to rhodopsin, the alpha subunit of the G protein replaces a molecule of GDP with a molecule of GTP and becomes activated. This replacement causes the alpha subunit of the G protein to dissociate from the beta and gamma subunits of the G protein. As a result, the alpha subunit is now free to bind to the cGMP phosphodiesterase (an effector protein).

Once enough PAR has accumulated, it will translocate from the nucleus into the cytosol. One study has suggested that PAR can translocate as a free polymer, however translocation of a protein-conjugated PAR cannot be ruled out and is in fact a topic of active research. PAR moves through the cytosol and enters the mitochondria through depolarization. Within the mitochondria, PAR binds directly to the AIF which has a PAR polymer binding site, causing the AIF to dissociate from the mitochondria.

Homoserine dehydrogenase has an NAD(P)H cofactor, which then donates a hydrogen to the same carbon, effectively reducing the aldehyde to an alcohol. (Refer to figures 1 and 2). However, the precise mechanism of complete homoserine dehydrogenase catalysis remains unknown. The homoserine dehydrogenase- catalyzed reaction has been postulated to proceed through a bi-bi kinetic mechanism, where the NAD(P)H cofactor binds the enzyme first and is the last to dissociate from the enzyme once the reaction is complete.

In December 2005, Leeds Metropolitan University Students Union (LMUSU) members chose via ballot to dissociate from the paper. In the past, this had been a joint venture between the two universities, but after continued complaints of a Leeds University centred perspective, a referendum was called to decide whether LMUSU should retain its link with the paper and continue paying a small proportion toward the paper's expenses. Members voted to dissolve the link, and henceforth the paper is a solely Leeds University Union maintained enterprise.

When it is time for a cell to enter S phase, complexes of cyclin-dependent kinases (CDK) and cyclins phosphorylate Rb to pRb, allowing E2F-DP to dissociate from pRb and become active. When E2F is free it activates factors like cyclins (e.g. cyclin E and cyclin A), which push the cell through the cell cycle by activating cyclin- dependent kinases, and a molecule called proliferating cell nuclear antigen, or PCNA, which speeds DNA replication and repair by helping to attach polymerase to DNA.

Whether the chelate dissociated and resulted in 64Cu binding to the protein superoxide dismutase (SOD) in rat liver was investigated by utilizing a gel electrophoresis assay for the detection of SOD. It was shown that 64Cu did in fact dissociate from the TETA chelator and bound to SOD, and to a lesser extent, other proteins. This is caused either because chelate-biomolecules are not thermodynamically stable or are not kinetically stable. Kinetic stability is more central in the determination of in vivo stability than thermodynamic stability however.

The weak adenine- uracil bonds lower the energy of destabilization for the RNA-DNA duplex, allowing it to unwind and dissociate from the RNA polymerase. Stem-loop structures that are not followed by a poly-uracil sequence cause the RNA polymerase to pause, but it will typically continue transcription after a brief time because the duplex is too stable to unwind far enough to cause termination. Rho-independent transcription termination is a frequent mechanism underlying the activity of cis-acting RNA regulatory elements, such as riboswitches.

A relaxase (one of the proteins of the relaxosome) nicks the plasmid at the nic site of the oriT, catalyzing a trans- esterification reaction that transfers the 5' end of the DNA at the nic site to a tyrosine residue on the relaxase. Relaxase then moves in the 5' to 3' direction on the plasmid, unwinding the DNA in a helicase-like fashion, until it comes a full circle back to the oriT where the relaxase recognizes a termination domain that causes it to dissociate from the DNA.

Messenger RNA produced from the LMNA gene undergoes alternative splicing and is translated into lamins A and C. Lamin A undergoes farnesylation to attach a membrane anchor to the protein. This version of the protein is also referred to as prelamin A. Farnesylated prelamin A is further processed into mature lamin A by a metalloproteinase removing the last 15 amino acids and its farnesylated cysteine. This allows lamin A to dissociate from the nuclear envelope membrane and fulfill nuclear functions. Mutations causing laminopathies interfere with these processes on different levels.

AD addresses dynamic variations in the formation, topology, size, and/or operations of collaborative systems such as enterprise alliances; self-organizing agent teams; sensor clusters; modular systems, and others. The AD principle analyzes the conditions and timing for individual/teams of agents to associate with or dissociate from a collaborative network. The AD decisions are made at different levels in the entire network, by individual agents, sub-networks or clusters of agents, or among multiple net-works. The analysis includes several phases, from creation and execution to dissolution and support.

Laboratory studies have found that early life stress in rodents can cause phosphorylation of methyl CpG binding protein 2 (MeCP2), a protein that preferentially binds CpGs and is most often associated with suppression of gene expression. Stress-dependent phosphorylation of MeCP2 causes MeCP2 to dissociate from the promoter region of a gene called arginine vasopressin (avp), causing avp to become demethylated and upregulated. This may be significant because arginine vasopressin is known to regulate mood and cognitive behavior. Additionally, arginine vasopressin upregulates corticotropin-releasing hormone (CRH), which is a hormone important for stress response.

Fig. 4 Sic1 Phosphorylation Mechanism 1. In mechanism 1, Koivomagi proposes that the phosphorylated primary site immediately shifts over to another location so that another CDK site can be phosphorylated during the same binding event. Fig. 5 Sic1 Phosphorylation Mechanism 2. Koivomagi proposes that phosphorylated primary site does not dissociate from the complex so that the intermediate CDK sites are sequentially phosphorylated in a single binding event. Cks1-dependent multi-phosphorylation occurs in a processive or semi- processive manner, evidenced by the lack of intermediate Sic1 phosphorylation states in normal cells.

STATs then bind to the phosphorylated tyrosines on the receptor using their SH2 domains, and then they are tyrosine-phosphorylated by JAKs, causing the STATs to dissociate from the receptor. These activated STATs form hetero- or homodimers, where the SH2 domain of each STAT binds the phosphorylated tyrosine of the opposite STAT, and the dimer then translocates to the cell nucleus to induce transcription of target genes. STATs may also be tyrosine-phosphorylated directly by receptor tyrosine kinases - but since most receptors lack built-in kinase activity, JAKs are usually required for signalling.

Importin proteins bind their cargo in the cytoplasm, after which they are able to interact with the nuclear pore complex and pass through its channel. Once inside the nucleus, interaction with Ran-GTP causes a conformational change in the importin that causes it to dissociate from its cargo. The resulting complex of importin and Ran-GTP then translocates to the cytoplasm, where a protein called Ran Binding Protein (RanBP) separates Ran- GTP from importin. Separation allows access to a GTPase activating protein (GAP) that binds Ran-GTP and induces the hydrolysis of GTP to GDP.

Nuclear export roughly reverses the import process; in the nucleus, the exportin binds the cargo and Ran-GTP and diffuses through the pore to the cytoplasm, where the complex dissociates. Ran-GTP binds GAP and hydrolyzes GTP, and the resulting Ran-GDP complex is restored to the nucleus where it exchanges its bound ligand for GTP. Hence, whereas importins depend on RanGTP to dissociate from their cargo, exportins require RanGTP in order to bind to their cargo. A specialized mRNA exporter protein moves mature mRNA to the cytoplasm after post-transcriptional modification is complete.

Cycling probe technology utilizes a cyclic, isothermal process that begins with the hybridization of the chimeric probe with the target DNA. Once hybridized, the probe becomes a suitable substrate for RNase H. RNase H, an endonuclease, cleaves the RNA portion of the probe, resulting in two chimeric fragments. The melting temperature (Tm) of the newly cleaved fragments is lower than the melting temperature of original probe. Because the CPT reaction is isothermally kept just above the melting point of the original probe, the cleaved fragments dissociate from the target DNA.

MDC1 has anti-apoptotic properties by directly inhibiting the apoptotic activity of the tumor suppressing protein p53. DNA damage can induce apoptosis when the ATM kinase and Chk2 phosphorylate p53 on its Ser-15 and Ser-20 residues which activates p53 and stabilizes it by allowing it to dissociate from the E3 ubiquitin protein ligase MDM2. MDC1 can execute its anti-apoptotic activity by inhibiting p53 in two ways. The MDC1 protein can bind to the n-terminus of p53 through its BRC1 domain which blocks p53 transactivation domain.

Naloxone (also known as Narcan) is used to reverse opioid overdose caused by drugs such as heroin or morphine. Similarly, Ro15-4513 is an antidote to alcohol and flumazenil is an antidote to benzodiazepines. Competitive antagonists are sub-classified as reversible (surmountable) or irreversible (insurmountable) competitive antagonists, depending on how they interact with their receptor protein targets.. Reversible antagonists, which bind via noncovalent intermolecular forces, will eventually dissociate from the receptor, freeing the receptor to be bound again.Stevens, E. (2013) Medicinal Chemistry: The Modern Drug Discovery Process. pg.

As a result, these cells dissociate from neural folds, gain motility, and disseminate to various parts of the embryo, where they differentiate to many other cell types. Also, craniofacial crest mesenchyme that forms the connective tissue forming the head and face, is formed by neural tube epithelium by EMT. EMT takes place during the construction of the vertebral column out of the extracellular matrix, which is to be synthesized by fibroblasts and osteoblasts that encircle the neural tube. The major source of these cells are sclerotome and somite mesenchyme as well as primitive streak.

In the environment, titanium dioxide nanoparticles have low to negligible solubility and have been shown to be stable once particle aggregates are formed in soil and water surroundings. In the process of dissolution, water-soluble ions typically dissociate from the nanoparticle into solution when thermodynamically unstable. TiO2 dissolution increases when there are higher levels of dissolved organic matter and clay in the soil. However, aggregation is promoted by pH at the isoelectric point of TiO2 (pH= 5.8) which renders it neutral and solution ion concentrations above 4.5 mM.

The group is reported to have had direct ties to the Muslim Brotherhood and Hamas, the later has been proscribed as a terrorist group by the UK government since 2001. In the past, MAB openly identified itself as an Islamist movement. In MAB's Inspire newspaper, produced for the 28 September 2002 anti-war demonstration, an article on the MAB's “Historical Roots and Background” links it explicitly to the Islamist tradition of the Muslim Brotherhood. At the UK Stop the War Coalition conference in January 2003, the Alliance for Workers' Liberty moved a motion to dissociate from MAB.

Most bacterial cell walls are negatively charged, therefore most antimicrobial polymers must be positively charged to facilitate the adsorption process. The structure of the counter ion, or the ion associated with the polymer to balance charge, also affects the antimicrobial activity. Counter anions that form a strong ion-pair with the polymer impede the antimicrobial activity because the counter ion will prevent the polymer from interacting with the bacteria. However, ions that form a loose ion-pair or readily dissociate from the polymer, exhibit a positive influence on the activity because it allows the polymer to interact freely with the bacteria.

FFA1 is found in highest concentration in Islets of Langerhans, the endocrine portion of the pancreas. Activation of FFA1 results in an increase in cytosolic Ca2+ via the phosphoinositide pathway. When a free fatty acid docks on FFA1, the membrane protein becomes activated. This activation causes one of its subunits to dissociate from the receptor, which then activates phospholipase C (PLC) which is found in the cell membrane. PLC in turn hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2),which is also in the membrane, to diacyl glycerol (DAG) which stays in the membrane, and inositol 1,4,5-triphosphate (IP3), which enters the cytosol.

The ability of importins and exportins to transport their cargo is regulated by GTPases, enzymes that hydrolyze the molecule guanosine triphosphate (GTP) to release energy. The key GTPase in nuclear transport is Ran, which can bind either GTP or GDP (guanosine diphosphate), depending on whether it is located in the nucleus or the cytoplasm. Whereas importins depend on RanGTP to dissociate from their cargo, exportins require RanGTP in order to bind to their cargo. Nuclear import depends on the importin binding its cargo in the cytoplasm and carrying it through the nuclear pore into the nucleus.

Epinephrine binds to a receptor protein that activates adenylate cyclase. The latter enzyme causes the formation of cyclic AMP from ATP; two molecules of cyclic AMP bind to the regulatory subunit of protein kinase A, which activates it allowing the catalytic subunit of protein kinase A to dissociate from the assembly and to phosphorylate other proteins. Returning to glycogen phosphorylase, the less active "b" form can itself be activated without the conformational change. 5'AMP acts as an allosteric activator, whereas ATP is an inhibitor, as already seen with phosphofructokinase control, helping to change the rate of flux in response to energy demand.

Type 1 receptors phosphorylate R-Smads at two C-terminal serines, which are arranged in an SSXS motif. Smads are localized at the cell surface by Smad anchor for receptor activation (SARA) proteins, placing them in proximity of type 1 receptor kinases to facilitate phosphorylation. Phosphorylation of the R-Smad causes it to dissociate from SARA, exposing a nuclear import sequence, as well as promoting its association with a Co-Smad. This Smad complex is then localized to the nucleus, where it is able to bind their target genes, with the help of other associated proteins.

The binding of the FNR to the integral membrane proteins on the thylakoid membrane is enhanced under acidic conditions, so recruitment and binding of FNR to the thylakoid membrane may be a method of storing and stabilizing the enzyme in the dark when photosynthesis is not occurring. The chloroplast stroma varies from being slightly acidic in the dark to more alkaline in the light. Therefore, in the dark, more FNRs would be recruited and bound to the thylakoid membrane, and in the light, more FNRs would dissociate from the membrane and be free in the stroma.

Nonetheless, following the claims, the Mayor of London's senior advisor on policing, Lee Jaspar, described the Muslim Boys as a terrorist front "as tough to crack as the IRA". On New Years Eve in December 2004, PDC affiliate Solomon Martin (also known as Blacker) was shot dead in Thornton Heath. Members of PDC that were affiliated with Blacker would dissociate from PDC and form their own gang known as CFR (Certified Riderz or Corelone Family Riderz). Other former members of PDC would form PIF (Paid In Full), and Tanna, who came up with the name "PDC", went and formed the 031 Bloods.

It has been suggested that the rearrangement, after the dissociation of the N2 molecule, proceeds over a reactive nitrene intermediate. These intermediates would be quite similar to those that have been proposed to be key intermediates in the rearrangement reactions named after Hofmann and Curtius, but have since been considered unlikely. When subjecting the azide to a Brønsted acid, the protonation of the azide activates the basal nitrogen and lowers the bond strength to the adjacent one, so that the dissociation and expulsion of molecular nitrogen is eased. After the rearrangement the proton can then dissociate from the iminium ion to yield the imine.

The average size limit for uORFs that will escape NMD is approximately 35 amino acids. It also has been suggested that uORFs inhibit translation of the downstream gene by trapping a ribosome initiation complex and causing the ribosome to dissociate from the mRNA transcript before it reaches the protein-coding regions. Currently, no studies have reported the global impact of uORFs on translational regulation. The current CCDS annotation guidelines allow the inclusion of mRNA transcripts containing uORFs if they meet the following two biological requirements: # the mRNA transcript has a strong Kozak signal; # the mRNA transcript is either ≥ 35 amino acids or overlaps with the primary open reading frame.

JAKs associate with a proline-rich region in each intracellular domain that is adjacent to the cell membrane and called a box1/box2 region. After the receptor associates with its respective cytokine/ligand, it goes through a conformational change, bringing the two JAKs close enough to phosphorylate each other. The JAK autophosphorylation induces a conformational change within itself, enabling it to transduce the intracellular signal by further phosphorylating and activating transcription factors called STATs (Signal Transducer and Activator of Transcription, or Signal Transduction And Transcription). The activated STATs dissociate from the receptor and form dimers before translocating to the cell nucleus, where they regulate transcription of selected genes.

The enzymes that are inhibited include serine proteases, which play a role in the IL-1 degradation of proteoglycans and collagen; lysosomal enzymes that cause proteoglycans to dissociate from hyaluronic acid; elastase; metalloproteinases such as stromelysin, which degrade cartilage matrix proteins; collagenases such as cathepsin B1; and hyaluronidase. PSGAG inhibits the synthesis of prostaglandin E2, which is released upon joint injury and causes inflammation, increases the loss of proteoglycan, and reduces the threshold of pain receptors. Inhibiting the complement pathway further reduces inflammation, most likely by altering C-reactive protein. The inhibition of blood coagulation reduces resultant fibrinolysis, which would cause cell death and increase local inflammation.

In addition to its role as a calcium buffer, calsequestrin also regulates the release of calcium from the sarcoplasmic reticulum by directly modulating ryanodine receptors. When the concentration of calcium is low, calsequestrin monomers form a complex with the proteins triadin and junctin, which inhibit ryanodine receptors. However, at high calcium concentrations, calsequestrin forms polymers that dissociate from the ryanodine receptor channel complex, removing the inhibitory response and increasing the sensitivity of the ryanodine receptor to spontaneously releasing calcium. Decreased CASQ2 is also associated with high levels of calreticulin, a protein which among other roles regulates the reuptake of calcium into the sarcoplasmic reticulum by SERCA.

The ligand-induced assembly of the complete receptor complex contains two IFNGR1 and two IFNGR2 subunits, which bring into close juxtaposition the intracellular domains of these proteins together with the inactive Jak1 and Jak2 kinases that they associate with. In this complex, Jak1 and Jak2 transactivate one another and then phosphorylate IFNGR1, thereby forming a paired set of Stat1 docking sites on the ligated receptor. Two Stat1 molecules then associate with the paired docking sites, are brought into close proximity with receptor-associated- activated JAK kinases, and are activated by phosphorylation of the Stat1. Tyrosine-phosphorylated Stat1 molecules dissociate from their receptor tether and form homodimeric complexes.

There are two principal signal transduction pathways involving the G protein-coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G-protein by exchanging its bound GDP for a GTP. The G-protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).

A washout step in the assay will usually distinguish between non-competitive and irreversible antagonist drugs, as effects of non-competitive antagonists are reversible and activity of agonist will be restored. Irreversible competitive antagonists also involve competition between the agonist and antagonist of the receptor, but the rate of covalent bonding differs and depends on affinity and reactivity of the antagonist. For some antagonist, there may be a distinct period during which they behave competitively (regardless of basal efficacy), and freely associate to and dissociate from the receptor, determined by receptor-ligand kinetics. But, once irreversible bonding has taken place, the receptor is deactivated and degraded.

Furthermore, TGF beta receptor I will dissociate from endoglin soon after it phosphorylates its cytoplasmic tail, leaving TGF beta receptor I inactive. Endoglin is constituitively phosphorylated at the serine and threonine residues in the cytoplasmic domain. The high interaction between endoglin's cytoplasmic and extracellular tail with the TGF beta receptor complexes indicates an important role for endoglin in the modulation of the TGF beta responses, such as cellular localization and cellular migration. Endoglin can also mediate F-actin dynamics, focal adhesions, microtubular structures, endocytic vesicular transport through its interaction with zyxin, ZRP-1, beta-arrestin and Tctex2beta, LK1, ALK5, TGF beta receptor II, and GIPC.

Tryptophan is bound by a number of interactions: other aromatic amino acid residues such as tryptophan, phenylalanine, and histidine seemingly "sandwich" the substrate, the N-H group of tryptophan forms hydrogen bonding interactions with the peptide backbone of the enzyme, and the amino acid moiety of tryptophan interacts with nearby tyrosine and glutamate residues. It is thought that the regioselectivity of the enzyme is a consequence of the spatial orientation of the tryptophan, with C7 placed close to the end of the tunnel where the chlorinating agent exits. Multiple halogenations are prevented as the addition of a halogen atom to the substrate greatly increases its steric bulk, causing it to dissociate from the enzyme.

Gonocytes are large cells with a spherical euchromatic nucleus, two nucleoli and a surrounding, ring-like cytosol. Throughout the majority of their developmental period, gonocytes are structurally supported by the cytoplasmic extensions of surrounding Sertoli cells and are suspended by Sertoli cell nuclei from the basement membrane. Gonocytes are attached to Sertoli cells by gap junctions, desmosome junctions and a number of different cell adhesion molecules such as connexin 43, PB- cadherin and NCAM for regulation of cell-to-cell communication. Gonocytes dissociate from these junctions and migrate so that the basal side of the cell is in close proximity with the basement membrane, where they undergo phenotypic changes and take the appearance of spermatogonia.

Unbound AR is mainly located in the cytoplasm, like a typical steroid receptor, and is associated with a complex of heat shock proteins (HSP) through interactions with LBD. Androgens, either agonists or antagonists, position themselves in the ligand-binding pocket (LBP) of the cytosolic AR and bind to the LBD, see figure 2. The AR goes through a series of conformational changes and HSP dissociate from AR. The transformed AR undergoes dimerisation, phosphorylation and translocates to the nucleus. The translocated receptor then binds to the androgen-response elements (ARE) on the promoter of the androgen responsive gene, a consensus sequence located either upstream or downstream of the transcription start site (TSS) of AR target genes.

When tau proteins become hyperphosphorylated they dissociate from the microtubules to which they provide stability and are thought to polymerize into neurofibrillary tangles in the brain and contribute to the onset of Alzheimer’s disease (Janssens & Goris, 2001). The B subunit of a CAPP confers the ability to dephosphorylate hyperphosphorylated tau proteins (Janssens & Goris, 2001). Hyperphosphorylated tau can interact with the acidic face of the B subunit and allow the catalytic subunit to dephosphorylate the protein (Janssens & Goris, 2001). Treatment of neuronal cells with okadaic acid has been shown to cause tau neurofibrillary tangles, indicating that disruptions of the interaction between CAPP, tau and microtubules can lead to the onset of Alzheimer’s disease (Janssens & Goris, 2001).

The heterodimers of RXR with nuclear receptors other than RAR (i.e. TR, VDR, PPAR, LXR) bind to various distinct response elements on the DNA to control processes not regulated by vitamin A. Upon binding of retinoic acid to the RAR component of the RAR-RXR heterodimer, the receptors undergo a conformational change that causes co-repressors to dissociate from the receptors. Coactivators can then bind to the receptor complex, which may help to loosen the chromatin structure from the histones or may interact with the transcriptional machinery. This response can upregulate (or downregulate) the expression of target genes, including Hox genes as well as the genes that encode for the receptors themselves (i.e.

The transcriptional assembly of the 40S subunit precursor, sometimes referred to as the small subunit processome (SSU) or 90S particle happens in a hierarchical fashion – essentially a stepwise incorporation of the UTP-A, UTP-B, and UTP-C subcomplexes. These subcomplexes are made up of over 30 non ribosomal protein factors, the U3 snoRNP particle, a few Rps proteins, and the 35S pre-rRNA. Their exact role, though has not been discovered. The composition of the pre-40S particle changes drastically once cleavage at the U3 snoRNPA dependent sites (sites A0, A1, and A2) are made. This cleavage event creates the 20S pre-rRNA and causes ribosomal factors to dissociate from the pre-40S particle.

However, unlike other caspase substrate proteins, the fragments of P35 do not dissociate from the caspase after cleavage. Instead, the N-terminal, 10 kDa cleavage fragment remains bound to the caspase by a covalent, stable thioester bond between the cleavage residue D87 of P35 and the cysteine residue at the active site of the caspase. While the formation of a thioester intermediate between the aspartate of the substrate's recognition site and the cysteine of the caspase's active site is a normal event in caspase-mediated protein cleavage, the resulting bond is normally quickly hydrolysed so that the cleaved products can detach. In the case of P35, however, the caspase-substrate complex remains stable.

Attempting to dissociate from the terror of the Pol Pot era, yet to emphasize revolutionary continuity and authority the Khmer Rouge renamed their army to National Army of Democratic Kampuchea (NADK) in December 1979 followed by political reorganisation and the demotion of Pol Pot to an advisor in 1985. NADK forces consisted of former RAK troops, conscripts forcibly recruited during the 1978/79 retreat, personnel pressed into service during in-country raids or drawn from among refugees and new volunteers. Military observers and journalists estimated around 40,000 and 50,000 NADK combatants, which were considered to be "the only effective [anti-Vietnamese] fighting force". In 1987 the opinion that the NADK was "the only effective fighting force" opposing the Vietnamese was expressed by foreign observers.

Sensing this, group member Donnie Wahlberg led the group in coordinating this remix album, which fused the "harder" elements of hip-hop and urban dance into the New Kids' sound, resulting in No More Games, No Mas Juegos & No More Games/The Remix Album — with a significant portion of the album remixed by Robert Clivilles and David Cole (of C+C Music Factory fame). Also employed was a marketing tactic to release the album under the 'NKOTB' acronym. Since the youngest group member was now eighteen years old, and the rest were in their early twenties, they had arguably grown out of the New 'Kids' On The Block moniker that they rose to fame with. More significantly, it was an attempt to dissociate from the stigma that was attached to that name.

Most pharmaceutical drugs are small molecules which elicit a physiological response by "binding" to enzymes or receptors, causing an increase or decrease in the enzyme's ability to function. The binding of a small molecule to a protein is governed by a combination of steric, or spatial considerations, in addition to various non-covalent interactions, although some drugs do covalently modify an active site (see irreversible inhibitors). Using the "lock and key model" of enzyme binding, a drug (key) must be of roughly the proper dimensions to fit the enzyme's binding site (lock). Using the appropriately sized molecular scaffold, drugs must also interact with the enzyme non-covalently in order to maximize binding affinity binding constant and reduce the ability of the drug to dissociate from the binding site.

In a review of research on patients with various neurological disorders, Pannu et al. found that metamemory was affected by various neurological disorders, including Korsakoff's amnesia, frontal lobe injury, multiple sclerosis and HIV. Other disorders, such as temporal lobe epilepsy, Alzheimer's disease, and traumatic brain injury had mixed results, and disorders such as Parkinson's syndrome and Huntington's syndrome showed no effect. In their review, Pannu and Kaszniak reached 4 conclusions: > (1) There is a strong correlation between indices of frontal lobe function > or structural integrity and metamemory accuracy (2) The combination of > frontal lobe dysfunction and poor memory severely impairs metamemorial > processes (3) Metamemory tasks vary in subject performance levels, and quite > likely, in the underlying processes these different tasks measure, and (4) > Metamemory, as measured by experimental tasks, may dissociate from basic > memory retrieval processes and from global judgments of memory.

The N-terminal α-helix of RNase A (residues 3-13) is connected to the rest of RNase A by a flexible linker (residues 16-23). As shown by F. M. Richards, this linker may be cleaved by subtilisin between residues 20 and 21 without causing the N-terminal helix to dissociate from the rest of RNase A. The peptide-protein complex is called "RNase S", the peptide (residues 1-20) is called the "S-peptide" and the remainder (residues 21-124) is called the "S-protein". The dissociation constant of the S-peptide for the S-protein is roughly 30 pM; this tight binding can be exploited for protein purification by attaching the S-peptide to the protein of interest and passing a mixture over an affinity column with bound S-protein. [A smaller C-peptide (residues 1-13) also works.

At this point, the subunits of the G-protein dissociate from the receptor, as well as each other, to yield a Gα-GTP monomer and a tightly interacting Gβγ dimer, which are now free to modulate the activity of other intracellular proteins. The extent to which they may diffuse, however, is limited due to the palmitoylation of Gα and the presence of an isoprenoid moiety that has been covalently added to the C-termini of Gγ. Because Gα also has slow GTP→GDP hydrolysis capability, the inactive form of the α-subunit (Gα-GDP) is eventually regenerated, thus allowing reassociation with a Gβγ dimer to form the "resting" G-protein, which can again bind to a GPCR and await activation. The rate of GTP hydrolysis is often accelerated due to the actions of another family of allosteric modulating proteins called Regulators of G-protein Signaling, or RGS proteins, which are a type of GTPase-Activating Protein, or GAP. In fact, many of the primary effector proteins (e.g.

In Escherichia coli, a species of bacteria, DAHP synthase is found as three isoenzymes, each of which sensitive to one of the amino acids produced in the shikimate pathway. In a study of DAHP synthase sensitive to tyrosine in E. coli, it was determined that the enzyme is inhibited by tyrosine through noncompetitive inhibition with respect to phosphoenolpyruvate, the first substrate of the reaction catalyzed by DAHP synthase, while the enzyme is inhibited by tyrosine through competitive inhibition with respect to D-erythrose 4-phosphate, the second substrate of the reaction catalyzed by DAHP synthase when the concentration of tyrosine is above 10 μM. It was also determined that the enzyme is inhibited by inorganic phosphate through noncompetitive inhibition with respect to both substrates and inhibited by DAHP through competitive inhibition with respect to phosphoenolpyruvate and noncompetitive inhibition with respect to D-erythrose 4-phosphate. Studies of product inhibition have shown that phosphoenolpyruvate is the first substrate to bind to the enzyme complex, inorganic phosphate is the first product to dissociate from the enzyme complex.

Born in Avilés, Asturias, Mendi began playing as a senior with local CU Collado Villalba in the 2008–09 season, in Tercera División. In April 2009 he signed with Athletic Bilbao, returning to youth football.El Athletic de Bilbao ficha al cadete del CUC Villalba Sergio Mendiguchía, de 15 años (Athletic de Bilbao signs CUC Villalba's youth player Sergio Mendiguchía); El Faro del Guadarrama, 13 April 2009 Mendi also appeared with the reserves in the 2010–11 season, in Segunda División B. After appearing with Athletic's farm team in the 2011–12 season, Mendi suffered a syncope in early April 2012, being sidelined for the rest of the season.Un síncope aparta al jugador del Athletic Mendigutxia de la competición (A syncope set aside Athletic's player Mendigutxia from the competition); El Mundo, 3 April 2012 He rescinded his link with the Biscay side on 3 November, after failing to appear in 2012–13.Mendigutxia llega a un acuerdo para desvincularse del Athletic (Mendigutxia reaches agreement to dissociate from Athletic); El Mundo Deportivo, 3 November 2012 On 30 January 2013 Mendi signed with La Roda CF, in the third level.