How to use circularizes in a sentence? Find typical usage patterns (collocations)/phrases/context for "circularizes" and check conjugation/comparative form for "circularizes". Mastering all the usages of "circularizes" from sentence examples published by news publications.
In: Neidhardt, FC et al. (1996) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (ASM Press, Washington, DC) These two single-stranded segments are the "sticky ends" of what is called the cos site. The cos site circularizes the DNA in the host cytoplasm. In its circular form, the phage genome, therefore, is 48,502 base pairs in length.
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This is distinct from HSV's equiprobable distribution, and the discriminatory mechanism is not known. A small percentage of isolated molecules are circular genomes, about which little is known. (It is known that HSV circularizes on infection.) There are at least 70 open reading frames in the genome. There are at least five clades of this virus.
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Latent Membrane Protein 2 (LMP2) is a rightward transcribing gene. LMP2’s transcript originates across the fused terminal repeats in sequences at opposite ends of the genome. hours after infection, the genome circularizes and the open reading frame is created. 1.7 kb and 2.0 kb messages are created by alternative promoter usage and differ only in the sequences of the first exon.
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Similarly, the EsV-1 phaeovirus has a linear double-stranded DNA genome with inverted repeats that have almost perfect homology. These inverted repeats could facilitate effective circularization of the genome and for a period of time it has been suspected that EsV-1 has a circular genome. The EhV-86 coccolithovirus is suggested to have both linear and circular genomes at different phases during DNA packaging. PCR amplification reveals random A/T overhangs, detection of DNA ligases and endonucleases hinting that a linear genome may be packaged and circularizes during DNA replication.
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Phages like the lambda phage use their site specific recombinases to integrate their DNA into the host genome during lysogeny. P1 phage DNA on the other hand, exists as a plasmid in the host. The Cre-lox system serves several functions in the phage: it circularizes the phage DNA into a plasmid, separates interlinked plasmid rings so they are passed to both daughter bacteria equally and may help maintain copy numbers through an alternative means of replication. The P1 phage DNA when released into the host from the virion is in the form of a linear double stranded DNA molecule.
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Little is known about the replication mechanisms of dicistroviruses but it is likely that they use a mechanism that is very similar to picornaviruses. The general picornavirus replication mechanism begins with the cloverleaf-shaped structure at the 5’ end of the RNA genome is bound by the 3CD protein. 3CD functions as an RNA-dependent RNA-polymerase. 3CD then interacts with another protein that binds the poly(A) tail. This circularizes the RNA and allows RNA polymerase to generate negative-sense RNA from the 3’ end while also being able to generate positive-sense RNA from the 5’ end. Translation of the genome is regulated by the binding of 3CD initially to the 5’ UTR.
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Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop.
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