129 Sentences With "nicking" | Random Sentence Generator

Other procedures include pricking or nicking the clitoris or clitoral hood.

A history of failure The practice of "ritual nicking" is not new.

Although, I guess bands nicking shit from other bands is hardly anything new.

But some communities practice less invasive rituals such as pricking or nicking the clitoris.

Other procedures, more common in parts of Asia, include nicking or pricking the clitoris.

This incident of people conveniently nicking Hello Kitty-themed items is not an isolated case.

TFA wound up with a 93% fresh rating, nearly nicking Empire (still tops, at 94%).

In Asia and the Middle East, the ritual may involve pricking or nicking the clitoris instead.

Hillary Clinton came close to nicking the vote there from Donald Trump last November, but fell short.

Swiping posh vehicles for resale and export is more difficult than nicking them off the street for joyriding.

Nicking your dad's black cashmere scarf for that 2K6 Windows movie-maker hood video was pretty mental, true.

Rooney employs this artery-nicking style while writing about love and lust among damaged and isolated and yearning young people.

"He's nicking money from me," said Roger Coquillaud, 65, a retired army officer at the modest central cafe in Civray.

Freeland (13-7) retired the first 11 Chicago batters he faced before nicking Jose Abreu with a pitch in the fourth.

When the school abruptly closed — John died of tetanus at 26 after nicking himself with a razor — Thoreau kept the desk.

I'm from Sudan and in my mother's generation, the Pharaonic version of FGM, the "ritual nicking," was reintroduced in Egypt and Sudan.

Items that they used to steal, such as televisions and car radios, have fallen in value so nicking them is no longer worthwhile.

I didn't get there in time for "No Place Like Om" but I'm nicking that name for when I retire to open a tea shop.

Earlier this season they had four players dismissed during a match – one of them for nicking the ref's red card and pretending to send him off.

It helped that my old man was a bit of a dresser, as I started nicking his stuff to wear and it snowballed from that really.

I was probably about 14, nicking fags out of my mother's packet, sneaking into the local pub, standard, and just trying to be like Mike really.

To mark the close of this beloved epoch with Star Wars:The Rise of Skywalker, the cast have been, well, casually nicking objects from set to keep as souvenirs.

Featuring a slowed-down 2-step beat and little flutterings of tropical hooks, it takes a certain generation back to nicking their parents' card details to download Donkey Kong.

So it gets a pass for accuracy, but loses several points for nicking someone else's life story.) Sue (Linda Kozlowski) is subjected to a fair amount of sexism, both intentional and unintentional.

But think about the pain of nicking your pits while shaving, or worse, the eye-watering sting of having all the hairs ripped out from the root at once by a wax.

Gone were the shin-nicking wooden frames, and the early sloshings, as water beds went waveless and mainstream, encased in soft-edged mattress forms that looked just like their coil-filled cousins.

"The sixth end she made a fantastic shot for two and then in the seventh end of course coming up slightly light on that draw and nicking the guard was crucial," Muirhead said.

From being chased and gently reprimanded for nicking strawberries as a kid to buying 50p mascara and developing conjunctivitis as a teenager, my magpie eyes were always drawn to the delights of Ridley Road.

On dirt, the ovals are short — often a half-mile around, about a quarter the size of most asphalt tracks — which means cars cluster nose to tail, nicking and bumping one another off the road.

We use this time to alternate between frothy-mouthed lust and aggression while pissing on everything we can and stealing drinks from bars and nicking hotel fixtures and/or religious icons because we think it's funny.

You may think you're shaving your legs by the book, but if you find yourself constantly nicking your skin, scratching ingrown hairs, and finding patches of hair here and there, your method could use a little work.

Only a brief change in the breeze could've sent the umbrella's big pole flying through her gut or one of the tips nicking a major artery, and the whole thing could've gone straight to Final Destination territory.

Back in 2013, a New Orleans man named Josh swallowed the toe that was being used for the cocktail, left $500 cash (the fine at the time for nicking the toe, before it was increased to $2,500), and split.

It is being fueled by a pervasive sense that Mr. Macron is pushing too far, too fast in too many areas — nicking at the benefits of pensioners and low earners, giving dollops to the well-off and slashing sacred worker privileges.

"There was nothing on the radio that sounded like it," he tells me in conversation last week about his 1993 debut single "Oh Carolina," a slow-rolling, "Peter Gunn"-nicking growler that also counted as the Kingston reggae veteran's first real-deal hit.

The bench clearings began innocently enough — with Severino nicking Josh Donaldson with a fastball in the first inning — but they escalated quickly enough because of deep roots: Jose Bautista's getting hit in the back on Friday, Marcus Stroman's jawing at the Yankees on Saturday, and two seasons' worth of domination by the flamboyant Blue Jays.

If you replace the outside edge of these polygons with a curved one and then cut those slices in half like before, you can create the new tiling solutions: The authors also showed that you can solve the problem by nicking a few triangles out these shapes, as long as they stay symmetrical: "I've no idea whether there are or ever will be any applications," Haddley said.

Henry Rollins may have never joined a band if it wasn't for lead singer H.R. stage-diving onto him; The Beastie Boys wouldn't have had the guts to transition from punk to hip-hop had they not witnessed Bad Brains' subversion of racial stereotypes; the drums on "Smells Like Teen Spirit" would've lacked their punch were it not for Dave Grohl nicking specific rhythms and fills from Earl Hudson.

It started when a gang, peacefully nicking stuff, was rudely disturbed by French rozzers.

Clearly, quenchable free radicals are the primary source of plasmid nicking in the purified solutions.

Also reported by Paul Hales, "Nicking folks' identities is easy, says researcher", The Inquirer, 24 March 2005.

Most bosses these days are Big Bad Swains, literally, not just metaphorically, nicking their top possie in the food chain.

It also inhibits the nicking-closing activity on the subunit of DNA gyrase that releases the positive binding stress on the supercoiled DNA.

A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved. They can be used for strand-displacement amplification, Nicking Enzyme Amplification Reaction, exonucleotyic degradation, the creation of small gaps, or nick translation. The latter process has been successfully used to incorporate both radioactively labelled nucleotides and fluorescent nucleotides allowing specific regions on a double stranded DNA to be studied.

Over 200 nicking enzymes have been studied, and 13 of these are available commercially and are routinely used for research and in commercial products.

The IGR contains the packing signal (PS) as well as sequences that dictate termination, nicking for replication, and the binding of II and IHF.

RecBCD mutants lacking detectable exonuclease activity retain high Chi hotspot activity in cells and nicking at Chi outside cells. A Chi site on one DNA molecule in cells reduces or eliminates Chi activity on another DNA, perhaps reflecting the Chi-dependent disassembly of RecBCD observed in vitro under conditions of excess ATP and nicking of DNA at Chi. Under both reaction conditions, the 3' strand remains intact downstream of Chi.

HUH endonucleases are broadly split into two categories of enzymes: replication initiator proteins (Rep) or relaxase / mobilization proteins. They both contain small protein domains that recognize sequence-specific origins of replication or origin of transfer at which site they nick DNA. The nicking domain of Reps tend to be smaller, on the order of 10-20 kDa while nicking domains from relaxases are larger, roughly 20-40 kDa in size.

There is disagreement among growers on whether nicking the seed coat is more harmful or helpful to germination and some support nicking,How to Grow Cochliasanthus caracalla from Seed. "by Krystal Miller, Demand Media" while others recommend against it. One widespread practice that does yield a high degree of success is to pour boiling water on the seeds and let the seeds soak overnight in that water as it naturally cools to room temperature.

The Nicking House is a historic building located in Iowa City, Iowa, United States. Henry C. Nicking, who was a barber, had this house built in 1854. It is one of the oldest houses in the city, and one of a very few that was constructed using sandstone. with The general architectural style is a stripped down version of the Greek Revival style, but a rear addition gives it a saltbox appearance.

Zinc-finger nickases (ZFNickases) are created by inactivating the catalytic activity of one ZFN monomer in the ZFN dimer required for double-strand cleavage. ZFNickases demonstrate strand-specific nicking activity in vitro and thus provide for highly specific single-strand breaks in DNA. These SSBs undergo the same cellular mechanisms for DNA that ZFNs exploit, but they show a significantly reduced frequency of mutagenic NHEJ repairs at their target nicking site. This reduction provides a bias for HR-mediated gene modifications.

Nb.BbvCI is a nicking endonuclease used to cut one strand of double-stranded DNA. It has been successfully used to incorporate fluorochrome-labeled nucleotides into specific spots of a DNA sequence via nick translation.

In the third round Dennis Wilshaw bagged a hat trick as Oldham Athletic were well beaten 5–1 before Ipswich Town came and frustrated Stoke and in the fourth round before nicking a 1–0 win.

Nicking Enzyme Amplification Reaction (NEAR) is a method for in vitro DNA amplification like the polymerase chain reaction (PCR). NEAR is isothermal, replicating DNA at a constant temperature using a polymerase (and nicking enzyme) to exponentially amplify the DNA at a temperature range of 55 °C to 59 °C. One disadvantage of PCR is that it consumes time uncoiling the double- stranded DNA with heat into single strands (a process called denaturation) . This leads to amplification times typically thirty minutes or more for significant production of amplified products.

Loop-mediated isothermal amplification is a similar idea, but done with a strand-displacing polymerase. Nicking enzyme amplification reaction (NEAR) and its cousin strand displacement amplification (SDA) are isothermal, replicating DNA at a constant temperature using a polymerase and nicking enzyme. Recombinase Polymerase Amplification (RPA) uses a recombinase to specifically pair primers with double-stranded DNA on the basis of homology, thus directing DNA synthesis from defined DNA sequences present in the sample. Presence of the target sequence initiates DNA amplification, and no thermal or chemical melting of DNA is required.

A half sheared sheep. Shearing can be done with use of hand-shears or powered shears. Professional sheep shearers can shear a sheep in under a minute, without nicking the sheep. The fleece is removed in one piece.

Then the 5' hydroxyl end of the broken DNA strand can reverse the phosphotyrosyl bond, enabling the release of TOP1 and religation of the DNA. The nicking and closing reactions are fast, and about 100 cycles can occur per second.

Arteriovenous nicking, also known as AV nicking, is the phenomenon where, on examination of the eye, a small artery (arteriole) is seen crossing a small vein (venule), which results in the compression of the vein with bulging on either side of the crossing. This is most commonly seen in eye disease caused by high blood pressure (hypertensive retinopathy). It is thought that, since the arteriole and venule share a common sheath, the arteriole's thicker walls push against those of the venule forcing the venule to collapse. This makes the venule form an hourglass shape around the arteriole.

DC Kezia Walker has a passion in life – nicking villains. She is a natural thief taker and a gutsy, working-class girl. She was adopted and brought up in a care home. Kezia arrives at Sun Hill fresh out of uniform, and still on her trainee period.

Diphthamide # Processing ## The leader region is cleaved during secretion. ## Proteolytic nicking separates A and B subunits, which remain joined by disulfide bonds until they reach the cytosol. # The toxin binds to heparin-binding epidermal growth factor precursor (HB-EGF). # The complex undergoes endocytosis by the host cell.

Demers won a clear points verdict, however, the decision was met with a chorus of boos with commentators stating that the fight was much closer than the score cards suggested with Demers just nicking many of the rounds. Demers stated "I thought I won the rounds, but closely!".

In a ring configuration this domain interacts with the ATP binding pocket of the neighboring subunit. The atomic structure of the HuH endonuclease domain of HBoV1 NS1 closely resembles the nickase structures encoded by other parvoviruses and by more-distant RCR replicons. This structure also mediates site-specific duplex DNA-recognition, which allows NS1 to bind site- specifically to viral replication origins positioned at each end of its genome (derived from the sequences of the viral hairpin telomeres). Origin recognition, which for some parvoviruses must be enhanced by the binding of additional cellular cofactors, leads to strand- and site-specific nicking of viral duplex DNA replication intermediates, processes that require ATP for tight binding and subsequent nicking.

Type IV is "[a]ll other harmful procedures to the female genitalia for non-medical purposes", including pricking, piercing, incising, scraping and cauterization. It includes nicking of the clitoris (symbolic circumcision), burning or scarring the genitals, and introducing substances into the vagina to tighten it.WHO 2008, 24.UNICEF 2013, 7.

Wilson, now angered, then turned and headed back toward Moore and a loud argument broke out. Moore eventually drew the pistol and shot Wilson twice, with one bullet striking his groin and the other nicking his abdominal aorta. Moore and Dixon fled the scene, with Moore seeking refuge at Murphy's home.

Hypertensive retinopathy with AV nicking and mild vascular tortuosity Hypertensive retinopathy is a condition characterized by a spectrum of retinal vascular signs in people with elevated blood pressure. It was first described by Liebreich in 1859.Liebreich R. Ophthalmoskopischer Befund bei Morbus Brightii. Albrecht von Graefes Arch Ophthalmol 1859; 5: 265–268.

Quaresma scored two goals in the first 10 minutes. For the first goal, Quaresma scored from a penalty shot in the third minute after Manuel Neuer tripped Martínez inside the penalty area. Neuer received a yellow card on the play. For his second goal in the 10th minute, Quaresma "nicking the ball from Dante" to set-up the goal.

In June 2019, scientists reported that all 16 GB of Wikipedia have been encoded into synthetic DNA. The first article describing data storage on native DNA sequences via enzymatic nicking was published in April 2020. In the paper, scientists demonstrate a new method of recording information in DNA backbone which enables bit-wise random access and in-memory computing.

The diagram shows the effects of nicks on intersecting DNA in a twisted plasmid. Nicking can be used to dissipate the energy held up by intersecting states. The nicks allow the DNA to take on a circular shape.Chao Ji, Lingyun Zhang, and Pengye Wang, "Configuration Transitions of Free Circular DNA System Induced by Nicks," Journal of Nanomaterials, vol.

Bodacious burst out of the chute, and then he jumped to the side once. Then he charged forward, which sent his rear legs over Custer's head. "For a moment, it looked as if Custer could withstand the force", but then he was thrown, a horn nicking his cheek. Custer only made two seconds on the bull.

It stretches the muscles to keep the tail in position, and is not used after the horse is retired from competition. Sometimes the process is sped up by the controversial practice of nicking or cutting the check ligament that normally pulls the tail downward. This practice is generally only used for a few breeds, such as the American Saddlebred.

The GyrA subunit is involved in the DNA nicking and ligation activity. Novobiocin has been shown to weakly inhibit the C-terminus of the eukaryotic Hsp90 protein (high micromolar IC50). Modification of the novobiocin scaffold has led to more selective Hsp90 inhibitors. Novobiocin has also been shown to bind and activate the Gram- negative lipopolysaccharide transporter LptBFGC.

This is naturally accomplished during fires, when the heat breaks the tough outer layer of the seed. When plants are propagated by hand, the seeds must be scarified by nicking the outer surface with a blade or soaking them in acid or boiling water. A conservation plan for the species will include establishing a normal fire regime for the habitat.

The fruit is green and cup-like when immature and woody, dehiscent capsule at maturity. Seeds are small and angular, remaining inconspicuous on the ground upon release. Seed coat/covering or testa of some Baeckea species has been recorded to form a physical barrier inhibiting seed germination. This may be reverted by removing or nicking the testa using a needle or scalpel, improving the rate of germination.

SR 91 begins at an intersection with U.S. Route 1 (US 1) in York. The highway proceeds out of town to the northwest towards South Berwick, just nicking the corner of Eliot along the way. The first of the route follow the York River towards South Berwick. SR 91 passes under Interstate 95 (the Maine Turnpike) without an interchange, then has its northern terminus at SR 236.

It was originally called E. coli DNA Helicase I, but is now known as F-plasmid TraI. In addition to being a helicase, the 1756 amino acid (one of the largest in E. coli) F-plasmid TraI protein is also responsible for both specific and non- specific single-stranded DNA binding as well as catalyzing the nicking of single-stranded DNA at the origin of transfer.

Also she drops a modern pistol that goes off, nicking Lars on the leg and destroying the coffee machine. Alfhildr tracks down a Beforeigner who raped her at age 12 and beats him soundly. Lars threatens to report her for police brutality but backs down when she says she will report his time-drops addiction. Since time migrants pop up in the bay, drownings are common.

National Geographic Oct. 1995, page 161 Although consumed as snacks, fruits constitute a major part of the food ingested by children and women looking after cattle as well as morans in the wilderness. The mixing of cattle blood, obtained by nicking the jugular vein, and milk is done to prepare a ritual drink for special celebrations and as nourishment for the sick.The Last of the Maasai.

PC Gemma Osbourne always wanted to be a cop. She served her two years' probation with Greater Manchester Constabulary then transferred to the Met expecting bright lights but getting leafy Surrey. Feeling isolated and home sick, she discovered a flair for driving and a fondness for nicking disorderly motorists. After four years on a response team, she successfully applied to become a 'black rat' and joined Met 315 Traffic Patrol.

Various techniques are used to numb the tail, most carrying significant health risks. Initially, tail-nicking was used to make the tail lay flat. However, this left a telltale scar. Next, mechanical means such as use of heavy rubber bands to constrict circulation in the tail were used, but these also left marks and could cause visible, permanent damage to the skin, hair and nerves of the tail.

Cos sequences are ~200 base pairs long and essential for packaging. They contain a cosN site where DNA is nicked at each strand, 12 bp apart, by terminase. This causes linearization of the circular cosmid with two "cohesive" or "sticky ends" of 12bp. (The DNA must be linear to fit into a phage head.) The cosB site holds the terminase while it is nicking and separating the strands.

In 2008 several UN bodies recognized FGM as a human-rights violation,WHO 2008, 8. and in 2010 the UN called upon healthcare providers to stop carrying out the procedures, including reinfibulation after childbirth and symbolic nicking. In 2012 the General Assembly passed resolution 67/146, "Intensifying global efforts for the elimination of female genital mutilations".UN resolution, 20 December 2012; Emma Bonino, "Banning Female Genital Mutilation", The New York Times, 19 December 2012.

The company was established in 1974 by Donald Comb as a cooperative laboratory of experienced scientists and initially produced restriction enzymes on a commercial scale. The company then began producing solution-oriented products. It received approximately $1.7 million in Small Business Innovation Research (SBIR) grants between 2009 and 2013 for this research. NEB produces 230 recombinant and 30 native restriction enzymes for genomic research, as well as nicking enzymes and DNA methylases.

Overdrying of DNA can also break the phosphodiester bond in DNA and result in nicks. Nicking endonuclease enzymes can assist with this process. A single-stranded break (nick) in DNA can be formed by the hydrolysis and subsequent removal of a phosphate group within the helical backbone. This leads to a different DNA conformation, where a hydrogen bond forms in place of the missing piece of the DNA backbone in order to preserve the structure.

Nicking endonucleases introduce the strand discontinuities, or DNA nicks, for both respective systems. Mut L homologues from eukaryotes and most bacteria incise the discontinuous strand to introduce the entry or termination point for the excision reaction. Similarly, in E. coli, Mut H nicks the unmethylated strand of the duplex to introduce the entry point of excision. For eukaryotes specifically, the mechanism of DNA replication elongation between the leading and lagging strand differs.

The day started poorly for them with Justin Langer falling for 14 on the seventh ball of the day, nicking a ball delivered by Hoggard behind to Geraint Jones. Ponting narrowly survived being run out early on and this proved crucial in the context of the match as the momentum gradually swung in Australia's direction. At one point Australia racked up runs at such a rate that a win became a real possibility.

Hathaway's original name was Jameson Babcock, and he grew up in Chester, Pennsylvania. In his youth, he was given the nickname Nick, which is short for "nickname." When he started writing songs, the name clicked, because he got most of his ideas by borrowing (or "nicking") from songwriters with talent. His stage name Hathaway came from the label of one of his shirts or a shirt he borrowed (or "nicked") from a friend.

Since 2010, the United Nations has called upon healthcare providers to stop performing all forms of the procedure, including reinfibulation after childbirth and symbolic "nicking" of the clitoral hood,UN 2010; . with intensified advocacy in the form of the Sustainable Development Goal 5 aimed at eliminating the harmful practice. The opposition to the practice is not without its critics, particularly among anthropologists, who have raised difficult questions about cultural relativism and the universality of human rights.; .

After the request is granted and some on-the-job training in being Liz's handler, Don breaks the news about the request to David. Don asks Charlie to help him during a home invasion case. Charlie's results confirm the area that the suspects would hit next. While checking out the property after a gun battle with the four suspects, a fifth man comes out and stabs Don, puncturing Don's left lung and nicking Don's pulmonary artery.

West of Granite Pass, the trail was in the Great Basin drainage. Rainfall in the Great Basin either flowed to the Humboldt River, sank into the ground or evaporated. The trail then jogged northwest until it reached Goose Creek where it headed southwest, nicking the far northwest corner of Utah and on into the future state of Nevada. The trail then headed southwest, down Goose Creek for about until it hit Thousand Springs Valley's creeks and springs.

Conjugative replication may require a second nick before successful transfer can occur. A recent report claims to have inhibited conjugation with chemicals that mimic an intermediate step of this second nicking event. 1.The insertion sequences (yellow) on both the F factor plasmid and the chromosome have similar sequences, allowing the F factor to insert itself into the genome of the cell. This is called homologous recombination and creates an Hfr (high frequency of recombination) cell. 2.

UNICEF 2013, 2. There is a high concentration in Indonesia, where according to UNICEF Type I (clitoridectomy) and Type IV (symbolic nicking) are practised; the Indonesian Ministry of Health and Indonesian Ulema Council both say the clitoris should not be cut. The prevalence rate for the 0–11 group in Indonesia is 49 percent (13.4 million). Smaller studies or anecdotal reports suggest that FGM is also practised in Colombia, Jordan, Oman, Saudi Arabia and parts of Malaysia;UNICEF 2013, 23.

Topoisomerase I is an enzyme that relaxes the strain on DNA by nicking and ligating the DNA. ATA inhibits the activity of this enzyme. Since this activity occurs in the nucleus of the cell ATA is a form of anti-nuclear antibody. Scleroderma results from the overproduction of collagen in affected tissues, one study claims that there is an increased density of Topoisomerase I sites in the collagen genes, and that the antibodies may be altering transcription at these loci.

A 1911 Bombay census of unknown reliability noted that they were performing clitorectomy. According to a 1991 article in Manushi, the Bohra remove either the clitoral hood or the tip of the clitoris. Supporters of the practice say that the Bohra remove only the clitoral hood or perform symbolic nicking, and that it should be referred to as "female circumcision", not FGM. A qualitative study in 2018 carried out by WeSpeakOut, a group opposed to FGM,Cantera, Angel L. Martínez (6 March 2018).

Colchester hosted Championship opposition in the first round of the League Cup, with Reading coming to the Community Stadium for the first time. The match saw Sam Walker make his first appearance of the season, but was replaced by Elliot Parish just before the hour mark following an injury. The match went to extra time, with Reading nicking a late goal through Chris Gunter to send the U's out of the competition in the first round for the fifth successive season.

The horse's tail is naturally raised without nicking or tail sets. Some classes allow special shoes that enhance action, and a relatively newer class allows the use of chains, six ounces and under as action devices. The practice of soring, illegal under the Horse Protection Act of 1970, is also seen within the Racking Horse world. Since the breed's inception, about 80,000 Racking Horses have been registered, with the largest populations located in the US states of Alabama and Tennessee.

The relaxosome is the complex of proteins that facilitates plasmid transfer during bacterial conjugation. The proteins are encoded by the tra operon on a fertility plasmid in the region near the origin of transfer, oriT. The most important of these proteins is relaxase, which is responsible for beginning the conjugation process by cutting at the nic site via transesterification. This nicking results in a DNA-Protein complex with the relaxosome bound to a single strand of the plasmid DNA and an exposed 3' hydroxyl group.

27, saying his brother intentionally ran him over. Judge LaBuda was eventually cleared of charges in connection with an ATV crash. Delaware County Family Court Judge Gary Rosa found that the charges - assault, menacing harassment and reckless endangerment in the initial encounter, and a separate charge of violating an order of protection - were not proven. According to excerpts from a Times Herald-Record story: On Sept. 25, near Wurtsboro, Frank LaBuda rode his ATV onto his brother’s property, allegedly “nicking” Peter Labuda as he passed.

Phage-T4 UV endonucleases also catalyze the reaction of the δ-reaction, nicking C5'-O-P bond at AP sites, although this reaction is slow and the enzyme should still be classified as AP lyase. This open ring allows the substitution of the correct base by other enzymes. DNA AP lyase activity is documented to have similar function in both E. Coli and in humans. A homolog of endonuclease III, human endonuclease III homolog 1, or hNTH1 functions similarly in humans as its homolog does in E. Coli.

Ionian's proprietary isothermal technology, termed the Nicking Enzyme Amplification Reaction Assay, is claimed to be capable of amplifying extremely low amounts of starting material to easily detectable levels in just a few minutes. The proprietary technology is based on the very rapid detection of small DNA or RNA fragments generated directly from the target nucleic acid. The amplification products can be detected by a variety of standard methods, including LC-MS, real-time fluorescence, lateral flow and capillary electrophoresis detection. Ionian's assay technology is therefore ideally suited for portable and handheld detectors and sensors.

He was bred by George Onett of Stone Gate Farm. Founder of Ocala Breeder Sales Company and first Chairman of the Board. sired by 1982 Canadian Hall of Fame inductee Runaway Groom, who in turn was a son of leading sire Blushing Groom. He was out of the mare Cherokee Dame, who is the daughter of Silver Saber, and Dame Franchesca who was by Frincis S. Onett popularized nicking to Nearco as with Cherikee Fellow and Groomstick wall with heavy traces directly back to Nearco, War Admiral, and Tom Fool.

Smichet was initially the aggressor and pushed forward relentlessly to take Demers out of his rhythm; however he lost momentum towards the end of the fight and Demers' superior technique pulled him through. Demers won a clear points verdict, however, the decision was met with a chorus of boos with commentators stating that the fight was much closer than the score cards suggested with Demers just nicking many of the rounds. Demers stated "I thought I won the rounds, but closely!". On 5 November 2011 Demers lost to Allan Green in Quebec City.

FGM awareness session run by the African Union Mission to Somalia at the Walalah Biylooley refugee camp, Mogadishu Common short-term complications include swelling, excessive bleeding, pain, urine retention, and healing problems/wound infection. A 2014 systematic review of 56 studies suggested that over one in ten girls and women undergoing any form of FGM, including symbolic nicking of the clitoris (Type IV), experience immediate complications, although the risks increased with Type III. The review also suggested that there was under-reporting. Other short-term complications include fatal bleeding, anaemia, urinary infection, septicaemia, tetanus, gangrene, necrotizing fasciitis (flesh-eating disease), and endometritis.

Due to a dispute with the record label, a new lineup moved to Bieler Bros. and has released six studio albums. In 2006, Webbe performed with Korn at the 2006 Download Festival, while Korn's lead singer Jonathan Davis was seriously ill, he sang A.D.I.D.A.S. He has also appeared on Bullet For My Valentine's 2008 album Scream Aim Fire (on "Take It Out On Me"), as well as Soulfly's debut album (on "Quilombo" and "Prejudice"). In 2015 Benji released his debut solo album, a purely reggae album entitled I Haven't Been Nicking In Ages, produced by Monsta Boy.

The game is unique in that it has no outlanes. Also, the game starts as 3-ball multiball with auto-plunger instead of a plunger; once the player started the game all three balls are released and objective is to knock down the set of drop targets in order to score points and build up time. Rather than counting balls, a player's score is determined based on the amount of time left on the timer which counts down when there's only 1 ball left. Disadvantages of this pinball machine include damage of the playfield and toys by multiple balls nicking each other.

The FBI converge on the building, and Olivia and Peter find Penrose about to cut into another victim. Olivia chases off after Christopher, who eventually succumbs to his rapid aging and dies, while Peter, after nicking Penrose with a bullet, confers with Walter to apply a makeshift defibrillator to bring the victim back to life. As they wrap up the case, Olivia, Peter, and Walter complete forms to finalize their position in the Fringe division. When Peter is out of earshot, Walter learns from Olivia that the FBI medical files on Peter's childhood are void of any details.

These molecules are then barcoded by restriction enzymes to allow for genomic localization through the technique of optical mapping. See the above section on "Technology" for those steps. Step 2: Template nicking DNase I is added to randomly nick the mounted DNA molecules. A wash is then performed to remove the DNase I. The mean number of nicks that occur per template is dependent on the concentration of DNase I as well as the incubation time. Step 3: Gap formation T7 exonuclease is added which uses the nicks in the DNA molecules to expand the gaps in a 5'–3' direction.

The lily is a favored organism for the cytological examination of meiosis since the chromosomes are large and each morphological stage of meiosis can be easily identified microscopically. Hotta et al. presented evidence for a common pattern of DNA nicking and repair synthesis in male meiotic cells of lilies and rodents during the zygotene–pachytene stages of meiosis when crossing over was presumed to occur. The presence of a common pattern between organisms as phylogenetically distant as lily and mouse led the authors to conclude that the organization for meiotic crossing-over in at least higher eukaryotes is probably universal in distribution.

English hopes of a win were high, and 20,000 people were locked out of the stadium in addition to the 23,000 capacity crowd. Australia started the day needing 399 runs from 98 overs if they were to claim an unlikely victory. The day started poorly for them with Langer falling for 14 on the seventh ball of the day, nicking a ball delivered by Matthew Hoggard behind to Geraint Jones. Ponting narrowly survived being run out early on and this proved crucial in the context of the match as the momentum gradually swung in Australia's direction.

Treatment requires both systemic oral treatment with most of the same drugs used in humans—terbinafine, fluconazole, or itraconazole—as well as a topical "dip" therapy. Detailed veterinary discussion of animal treatment Because of the usually longer hair shafts in pets compared to those of humans, the area of infection and possibly all of the longer hair of the pet must be clipped to decrease the load of fungal spores clinging to the pet's hair shafts. However, close shaving is usually not done because nicking the skin facilitates further skin infection. Twice-weekly bathing of the pet with diluted lime sulfur dip solution is effective in eradicating fungal spores.

An origin of transfer (oriT) is a short sequence (up to 500 bp) that is necessary for transfer of the DNA that contains it from a bacterial host to recipient during bacterial conjugation. The oriT is cis-acting — it is found on the same DNA that is being transferred, and it is transferred along with the DNA. The origin of transfer consists of three functionally defined domains: a nicking domain, a transfer domain, and a termination domain. At the beginning of conjugation of plasmids, a multi-protein complex called the relaxosome assembles around the oriT, with each individual protein binding at specific sites on the oriT.

Despite its increased efficacy, the edit inserted by PE2 might still be removed due to DNA mismatch repair of the edited strand. To avoid this problem during DNA heteroduplex resolution, an additional single guide RNA (sgRNA) is introduced. This sgRNA is designed to match the edited sequence introduced by the pegRNA, but not the original allele. It directs the Cas9 nickase portion of the fusion protein to nick the unedited strand at a nearby site, opposite to the original nick. Nicking the non-edited strand causes the cell’s natural repair system to copy the information in the edited strand to the complementary strand, permanently installing the edit.

Charlie, a man with an extraordinary physical resemblance to Ankh-Morpork Patrician Havelock Vetinari, ran a clothes shop there (The Truth). Nobby Nobbs had an early career as an armourer in one of the Pseudopolis city militias; curiously (and this may be no more than a coincidence, although there may be a connection given Nobby's penchant for nicking and flogging [i.e., stealing and selling] anything not nailed down), Pseudopolis always lost its wars at this period because of an unaccountable shortage of weapons. John Keel, a city watchman, moved from Pseudopolis to Ankh-Morpork and was the mentor of the young Sam Vimes (Night Watch).

He engaged in a short-lived flirtation with Registrar Diane Lloyd, but this too ended with him allowing Diane to take the blame for his own mistake - accidentally nicking a patient's urethra while in surgery. After expressing a desire to return to cardiothoracics, Nick impressed Lord Byrne - cardiothoracic head of Holby General's neighbouring hospital St Luke's - with his handling of a suicide bomb attack at a charity dinner. He was offered a Consultancy in his preferred specialty, and departed from Holby once more. Nick returned to Holby City Hospital for a third time in 2008, this time in Holby City's sister show Casualty, as Clinical Lead of the hospital's Emergency Department.

However, this method is still less drastic than in the past, when the tail was actually broken and then placed into the tail set, healing in the artificial position. Tail-breaking for high-set tails is no longer used, and tail-nicking is banned in a few states. In certain Iberian-descended gaited horse breeds, notably the Paso Fino, where a quiet, low-carried tail is desired, the low tail set was occasionally achieved by actually breaking the tailbone at a certain point so that it would remain in the desired position. The horse can still use its tail to some degree, but cannot keep it raised when in motion.

Yakubu et al., 2014 Removal of the seed coat, soaking in water for 72 hours, placing inside air-tight transparent polythene bags and sprinkling water on the seeds when needed for constant moisture gives an early germination period of two weeks, while the combination of freshly harvested seeds, pierced minimally and tied in black nylon reduces the germination period to between five and seven days as the piercing or nicking procedure makes for quick availability of water and oxygen to the seed embryo.Okonkwo, H. O., Koyejo, O. A., Osewa, S. O. and Babalola, O. T. (2014). Techniques for Improvement of Garcinia Kola (Heckel) Seeds Germination.

One of the most significant supporting characters of the comic strip, this nomadic Arab lives alone in the desert, and is never named. He was exiled from a local Tuareg tribe for 'nicking' (stealing), and will not be allowed back unless he proves himself by presenting the head of an enemy warrior. For this reason, the Nomad occasionally tries to take over the fort, often failing for stupid reasons, such as forgetting his ladder, forgetting that there is only one of him, or attempting to infiltrate the fort using ridiculous disguises. Beau Peep has outwitted the Nomad on numerous occasions, meaning he has become a mortal enemy of the Nomad.

The Miami News described the three "right on target" Stock Aitken Waterman songs as "pop gems that display Jackson at her best." "Just Say No" was commended as a "bouncy tune that glosses over" the anti-drugs slogan while AllMusic wrote that "Just Say No" brought "out the best of Jackson."[ AllMusic] David Quantick, writing in NME, lauded the LP's hip-hop side with Full Force "a-nicking and a-sticking in full sample effect." However Quantick slammed the SAW-produced tracks on Side B, characterizing "Just Say No" as Marvin Gaye's "What's Going On" rammed up the nose of ABBA's "I Have a Dream".

However everyone is shocked when a struggle reveals that the true One is actually Lindsay rather than Sarah, as the Knife of Truth ends up nicking her throat. They also find that the Knife can cut through anything if it has the blood of the One on it. Corrine immediately imprisons Lindsay in preparation for her initiation into the sorority and kills Trina Goodwin, as she is no longer of any use to Corrine. Sarah escapes with Finn and the two end up having sex in order to keep him from being sacrificed in a ceremony where the Alpha Nus will throw virgins into their Eternal Flame to keep it burning.

RPA is one of several isothermal nucleic acid amplification techniques to be developed as a molecular diagnostic technique, frequently with the objective of simplifying the laboratory instrumentation required relative to PCR. A partial list of other isothermal amplification techniques include LAMP, NASBA, helicase-dependent amplification (HDA), and nicking enzyme amplification reaction (NEAR). The techniques differ in the specifics of primer design and reaction mechanism, and in some cases (like RPA) make use of cocktails of two or more enzymes. Like RPA, many of these techniques offer rapid amplification times with the potential for simplified instrumentation, and reported resistance to substances in unpurified samples that are known to inhibit PCR.

The DSBR pathway is unique in that the second 3' overhang (which was not involved in strand invasion) also forms a Holliday junction with the homologous chromosome. The double Holliday junctions are then converted into recombination products by nicking endonucleases, a type of restriction endonuclease which cuts only one DNA strand. The DSBR pathway commonly results in crossover, though it can sometimes result in non-crossover products; the ability of a broken DNA molecule to collect sequences from separated donor loci was shown in mitotic budding yeast using plasmids or endonuclease induction of chromosomal events. Because of this tendency for chromosomal crossover, the DSBR pathway is a likely model of how crossover homologous recombination occurs during meiosis.

Rising to his feet, Phelan engages Andy in a brawl and gets the upper hand despite being pummeled multiple times. He then grabs the laptop and, recalling this story to have a different ending in contrast to "the bad guy getting his comeuppance", smashes Andy on the head with it — supposedly killing him. After sending a text to Steph from Andy's mobile phone to dump her, causing her to leave the country alone, Phelan goes on to meet Eileen at the wedding reception where they get married; with Todd and Billy present as well. In February 2017, Phelan catches Faye and her boyfriend Seb Franklin (Harry Visinoni) nicking his tools from his backyard.

Wood established his own group at ICRF and over the next decade, performed a series of biochemistry experiments to understand each step in the repair process by adding individual, purified proteins. Repair (particularly of UV-induced pyrimidine dimers)includes recognition of the damaged site (probably by sensing an unpaired bubble at the mutation site), nicking the DNA at upstream and downstream sites, excising the damaged DNA, then filling in the single- stranded DNA gap using a polymerase, with the opposite strand serving as a template for the proper sequence for the repair patch. Since multiple proteins are involved in NER, different XP patients may have different gene mutations (called "complementation groups" based on which enzyme is defective in the NER pathway).

According to the Dawoodi Bohra Women's Association for Religious Freedom, the study's conclusions did not reflect the views of most Bohra women. In Australia in 2018, the convictions of three members of the Bohra community, related to performing FGM on two girls, were overturned when the appeal court accepted that the tip of each girl's clitoris was still visible and had not been "mutilated"; the defence position was that only "symbolic khatna" had been performed.. The High Court of Australia overturned that decision in October 2019, ruling that the phrase "otherwise mutilates" in Australian law does encompass cutting or nicking the clitoris. As a result, the convictions were upheld, and the defendants received custodial sentences of at least 11 months. .

Growers can overcome this dormancy either by nicking the seed coat away from the 'eye' of the seed, by rubbing the seed gently between pieces of sandpaper, or by placing the seed in hot (just off-boiling) water and leaving it to soak overnight. Once germinated, seedlings quickly establish a deep taproot, vital for desert survival. This means that if domestically grown, they should either be planted in their intended final location, transplanted as soon as possible after germination, or grafted as a seedling on to a different root such as the bladder senna, Colutea arborescens. They do not tolerate disturbance of their roots but, once established in well-drained soil, require little and infrequent watering, and can withstand extreme heat and sunshine, as well as light frosts.

Relaxation is not an active process and energy (in the form of ATP) is not spent during the nicking or ligation steps; this is because the reaction between the tyrosine residue at the active site of the enzyme with the phosphodiester DNA backbone simply replaces one phosphomonoester bond with another. The topoisomerase also does not use ATP during uncoiling of the DNA; rather, the torque present in the DNA drives the uncoiling and proceeds on average energetically downhill. Recent single molecule experiments have confirmed what bulk-plasmid relaxation experiments have proposed earlier, which is that uncoiling of the DNA is torque-driven and proceeds until religation occurs. No data suggest that Topo IB "controls" the swiveling insofar as that it has a mechanism in place that triggers religation after a specific number of supercoils removed.

A major petty crime problem, drug addiction, and add to that people are fed up with bad housing." Shawnie, a 2006 novel by social worker Ed Trewavas, written from the point of view of a 13-year-old girl living in Knowle West, documents some of the deprivation he encountered in his work. One of the novel's characters "describes Knowle West ... as a 'shit hole' populated by 'yokels, cider-heads, junkies, dole-scammers, slappers and failed wide boys, all interbreeding and nicking their cruddy possessions off of each other in some giant, dismal rota.'" The Guardian in 2007 reported "Fed up with the media's view of their community as a hub for drug use, crime and antisocial behaviour, the residents of one of Britain's most notorious housing estates decided to fight back.

Douglas served as an officer in the Volunteer Force London Rifle Volunteer Brigade and was commissioned as an Ensign in that unit on 17 June 1861. He received promotion to Lieutenant in that unit, since renamed the London Rifle Volunteer Corps, on 18 November 1863. During this period Douglas held several patents including one for "improvements in machinery for nicking and dressing the heads of screw-blanks" which was approved on 28 April 1868, one for "improvements in the manufacture of method of repairing railway rails and other iron in a permanent way and in the machinery to be employed therein" on 30 July 1868 and one for "improvements in screw cutting and threading machines and in the construction of screw nails and spikes" on 30 April 1866. The latter two patents expired after a seven-year period for non-payment of stamp duty required for a patent extension.

These residues are critical for nicking the DNA strand and for forming the DNA hairpin. Residues 384–454 of RAG-1 comprise a nonamer- binding region (NBR) that specifically binds the conserved nonomer (9 nucleotides) of the RSS and the central domain (amino acids 528–760) of RAG-1 binds specifically to the RSS heptamer. The core region of RAG-2 is predicted to form a six-bladed beta-propeller structure that appears less specific than RAG-1 for its target. Cryo-electron microscopy structures of the synaptic RAG complexes reveal a closed dimer conformation with generation of new intermolecular interactions between two RAG1-RAG2 monomers upon DNA binding, compared to the Apo-RAG complex which constitutes as an open conformation. Both RAG1 molecules in the closed dimer are involved in the cooperative binding of the 12-RSS and 23-RSS intermediates with base specific interactions in the heptamer of the signal end.